STAG2 (D25A4) XP® Rabbit mAb #5882
- WB
- IP
- IF
- ChIP
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 141 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
- ChIP-Chromatin Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
For optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Immunofluorescence (Immunocytochemistry) | 1:100 - 1:400 |
| Chromatin IP | 1:50 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Source / Purification
Background
- Peters, J.M. et al. (2008) Genes Dev 22, 3089-114.
- Barbero, J.L. (2009) Cell Mol Life Sci 66, 2025-35.
- Hoque, M.T. and Ishikawa, F. (2001) J Biol Chem 276, 5059-67.
- Hauf, S. et al. (2005) PLoS Biol 3, e69.
- Hauf, S. et al. (2001) Science 293, 1320-3.
- Pati, D. et al. (2002) Mol Cell Biol 22, 8267-77.
- Chen, F. et al. (2002) J Biol Chem 277, 16775-81.
Limited Uses
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