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STOP (175) Mouse Monoclonal Antibody #4265

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  • IF

    Product Specifications

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 120
    Source/Isotype Mouse IgG1
    Application Key:
    • WB-Western Blotting 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunofluorescence (Frozen) 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    For a carrier free (BSA and azide free) version of this product see product #73152.

    Protocol

    Specificity / Sensitivity

    STOP (175) Mouse Monoclonal Antibody detects endogenous levels of total N-STOP and O-STOP proteins.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with purified N-STOP from rat brain. The antigen is encoded by exon 4 (Galiano et al., 2004).

    Background

    Stable Tubule Only Polypeptide (STOP) is a microtubule-associated protein, and its microtubule-stabilizing activity is regulated by calmodulin (1-2). STOPs have several tissue- and developmental-specific isoforms that are encoded by a single gene. Neurons express N-STOP (exons 1-4) and E-STOP (exons 1-3), fibroblasts express F-STOP (exons 1-2), oligodendrocytes express O-STOP, and astrocytes A-STOP (3). STOPs are the major contributors stabilizing microtubules that resist depolymerization due to cold or depolymerizing drugs. STOP knock-out mice display impaired synaptic plasticity associated with severe behavioral disorders in contrast to the anticipated neuronal development and brain anatomy defects (4).

    Alternate Names

    2810411E12Rik; F-S; MAP-6; Map6; Microtubule-associated protein 6; Mta; Mtap6; Stable tubule-only polypeptide; STOP

    For Research Use Only. Not for Use in Diagnostic Procedures.
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