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SUMO-2/3 Antibody #4974

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Inquiry Info. # 4974

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    Product Specifications

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa)
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50
    Flow Cytometry (Fixed/Permeabilized) 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    SUMO-2/3 Antibody detects endogenous levels of SUMO-2/3. This antibody does not cross-react with recombinant SUMO-1.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human SUMO-2/3. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Small ubiquitin-related modifier 1, 2 and 3 (SUMO-1, -2 and -3) are members of the ubiquitin-like protein family (1). The covalent attachment of the SUMO-1, -2 or -3 (SUMOylation) to target proteins is analogous to ubiquitination. This post-translational modification is a reversible, multi-step process that is initiated by cleaving a precursor protein to a mature protein. Mature SUMO-1, -2 or -3 is then linked to the activating enzyme E1, conjugated to E2 and in conjunction with E3, SUMO-1, -2 or -3 is ligated to the target protein (2). Ubiquitin and the individual SUMO family members are all targeted to different proteins with diverse biological functions. Ubiquitin predominantly regulates degradation of its target (1). In contrast, SUMO-1 is conjugated to RanGAP, PML, p53 and IκB-α to regulate nuclear trafficking, formation of subnuclear structures, regulation of transcriptional activity and protein stability (3-7). SUMO-2/-3 forms poly-(SUMO) chains, is conjugated to topoisomerase II and APP, regulates chromosomal segregation and cellular responses to environmental stress, and plays a role in the progression of Alzheimer disease (8-11).

    Alternate Names

    HSMT3; MGC117191; sentrin 2; Sentrin-2; small ubiquitin like modifier 2; small ubiquitin like modifier 3; small ubiquitin-like modifier 2; Small ubiquitin-related modifier 2; Small ubiquitin-related modifier 3; SMT3 homolog 1; SMT3 homolog 2; SMT3 suppressor of mif two 3 homolog 1; SMT3 suppressor of mif two 3 homolog 2; SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae); SMT3 suppressor of mif two 3 homolog 3; SMT3 suppressor of mif two 3 homolog 3 (S. cerevisiae); Smt3A; SMT3A; Smt3B; SMT3B; SMT3H1; SMT3H2; SUMO 3; SUMO-2; SUMO-3; SUMO2; SUMO3; ubiquitin-like protein SMT3A; Ubiquitin-like protein SMT3A; ubiquitin-like protein SMT3B; Ubiquitin-like protein SMT3B

    For Research Use Only. Not for Use in Diagnostic Procedures.
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