Western blot analysis of extracts from mouse and rat brain, and mouse pancreas using SYNGR1 Antibody (upper), and α-Actinin (D6A8) Rabbit mAb #6487 (lower).
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Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
NOTE: Please refer to primary antibody datasheet or product webpage for recommended antibody dilution.
From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: #12957 Western Blotting Application Solutions Kit
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm).
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
* Avoid repeated exposure to skin.
posted June 2005
revised November 2013
Reprobing of an existing membrane is a convenient means to immunoblot for multiple proteins independently when only a limited amount of sample is available. It should be noted that for the best possible results a fresh blot is always recommended. Reprobing can be a valuable method but with each reprobing of a blot there is potential for increased background signal. Additionally, it is recommended that you verify the removal of the first antibody complex prior to reprobing so that signal attributed to binding of the new antibody is not leftover signal from the first immunoblotting experiment. This can be done by re-exposing the blot to ECL reagents and making sure there is no signal prior to adding the next primary antibody.
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalently purified water.
posted June 2005
revised October 2016
Protocol Id: 10
SYNGR1 Antibody recognizes endogenous levels of total synaptogyrin-1 protein.Species Reactivity:
Human, Mouse, Rat
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr214 of human synaptogyrin-1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Synaptogyrin, or SYNGR, are a family of tyrosine-phosphorylated proteins, including neuronal SYNGR1 and SYNGR3 that are found in synaptic vesicles and contribute to the proper synapse function. Synaptogyrin-2 (SYNGR2) expresses ubiquitously and it is not only associated with synaptic vesicles, but also plays an important role in exocytosis processes (1,3). In addition, it has been shown that SYNGRs modulate calcium currents in excitable cells during potassium chloride-dependent exocytosis (3). SYNGR3 and SYNGR1 specifically localize in synaptic vesicles. SYNGR1 modulates synaptic vesicle function similar to SYNGR3 (2,3). SYNGR1 and SYNGR3 contribute to the neurotransmitter release in neurons by interactions with the GABA and VGLUT transporters in primary neurons and in C. elegans (4-6). SYNGRs are associated with disease including Schizophrenia (7,8) and Alzheimer's disease (9,10).
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