Syntenin-1/MDA9 (E2I9L) Rabbit mAb #27964
- WB
- IP
- IF
- F
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 30 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunofluorescence (Immunocytochemistry) | 1:100 - 1:400 |
| Flow Cytometry (Fixed/Permeabilized) | 1:50 - 1:100 |
Storage
For a carrier free (BSA and azide free) version of this product see product #32485.
Protocol
Specificity / Sensitivity
Syntenin-1/MDA9 (E2I9L) Rabbit mAb recognizes endogenous levels of total syntenin-1/MDA9 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human syntenin-1/MDA9 protein.
Background
Syntenin-1 (MDA9, SDCBP) is a member of the PDZ family of proteins (1), defined by the presence of a PDZ protein interaction domain. Syntenin-1, via its PDZ domain, functions as a scaffold adaptor protein that regulates numerous signal transduction pathways, including FAK, Src, TGFβR1, EGFR, and IGF1R (2-5). Aberrantly elevated expression of syntenin-1 has been found in various cancers, and has been linked to growth and invasion of tumor cells (5-7). In the central nervous system, syntenin-1 has been shown to mediate neuron dendrite protrusion and spine formation through its interaction with syndecan and Rheb (8). Syntenin-1 is also a key component of the syndecan/syntenin/ALIX pathway which has been shown to directly contribute to the formation and internalization of exosomes (9-11).
- Pradhan, A.K. et al. (2020) Cancer Metastasis Rev 39, 769-781.
- Dasgupta, S. et al. (2013) Clin Cancer Res 19, 4621-33.
- Das, S.K. et al. (2012) Front Biosci (Landmark Ed) 17, 1-15.
- Menezes, M.E. et al. (2016) Oncotarget 7, 80175-80189.
- Das, S.K. et al. (2018) Cancer Res 78, 2852-2863.
- Das, S.K. et al. (2019) Adv Cancer Res 144, 137-191.
- Kegelman, T.P. et al. (2015) Expert Opin Ther Targets 19, 97-112.
- Shimada, T. et al. (2019) Int J Mol Sci 20, 4171. doi: 10.3390/ijms20174171.
- Fares, J. et al. (2017) Cell Adh Migr 11, 124-126.
- Baietti, M.F. et al. (2012) Nat Cell Biol 14, 677-85.
- Kugeratski, F.G. et al. (2021) Nat Cell Biol 23, 631-641.
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