Cat. # | Size | Qty. | Price |
---|---|---|---|
14541T | 1 Kit (8 x 20 microliters) |
|
Product Includes | Quantity | Applications | Reactivity | MW(kDa) | Isotype |
---|---|---|---|---|---|
CD3ε (CD3-12) Rat mAb 4443 | 20 µl |
|
H M | 21 | Rat IgG1 |
Phospho-LAT (Tyr220) Antibody 3584 | 20 µl |
|
H | 36, 38 | Rabbit |
Phospho-Lck (Tyr505) Antibody 2751 | 20 µl |
|
H M | 56 | Rabbit |
Phospho-PLCγ1 (Tyr783) (D6M9S) Rabbit mAb 14008 | 20 µl |
|
H M | 155 | Rabbit IgG |
Phospho-Src Family (Tyr416) (D49G4) Rabbit mAb 6943 | 20 µl |
|
H M R Mk | 60 | Rabbit IgG |
Phospho-SLP-76 (Ser376) (D9D6E) Rabbit mAb 14745 | 20 µl |
|
H M | 76 | Rabbit IgG |
Phospho-Zap-70 (Tyr319)/Syk (Tyr352) (65E4) Rabbit mAb 2717 | 20 µl |
|
H M | 70, 72 | Rabbit IgG |
Phospho-Zap-70 (Tyr493)/Syk (Tyr526) Antibody 2704 | 20 µl |
|
H | 70 | Rabbit |
Anti-rat IgG, HRP-linked Antibody 7077 | 100 µl |
|
R | Goat | |
Anti-rabbit IgG, HRP-linked Antibody 7074 | 100 µl |
|
Goat |
Product Information
Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a region surrounding Pro184 of human CD3ε, and with a synthetic phosphopeptide corresponding to residues surrounding Tyr783 of human PLCγ1 protein, Tyr416 of human Src protein, Ser376 of human SLP-76 protein, and Tyr319 of human Zap-70 protein. Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Tyr220 of human LAT protein, Tyr505 of human Lck protein, and Tyr493 of human Zap-70 protein. Antibodies are purified by protein A and peptide affinity chromatography.
When T cells encounter antigens via the T cell receptor (TCR), information about the quantity and quality of antigens is relayed to the intracellular signal transduction machinery (1). This activation process depends mainly on CD3 (Cluster of Differentiation 3), a multiunit protein complex that directly associates with the TCR α and ß chains. CD3 is composed of four polypeptides: ζ, γ, ε and δ. Each of these polypeptides contains at least one immunoreceptor tyrosine-based activation motif (ITAM) (2). The Src family kinases Lck and Fyn are recruited to the TCR complex upon stimulation and activate the downstream tyrosine kinases to initiate signaling. Phosphorylation of Lck at Tyr394 leads to an increase in Lck activity while phosphorylation of Tyr505 in the Lck carboxy-terminal tail down-regulates Lck catalytic activity (3). Zap-70 and Syk are rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by Src family tyrosine kinases. Activation loop phosphorylation of Zap-70 at Tyr493 and Syk at Tyr526 leads to complete activation of both kinases (4). Subsequent phosphorylation of other tyrosine residues within the kinase interdomain B region, including Zap-70 at Tyr315 and Zap-70 at Tyr 319, create docking sites for downstream signaling molecules. Zap-70 and Syk phosphorylate the transmembrane adaptor protein LAT at multiple, conserved tyrosine residues within SH2 binding motifs, exposing these motifs as docking sites for downstream signaling targets (5,6). The phosphorylation of LAT at Tyr171 and Tyr220 enables the binding of Grb2, Gads/SLP-76, PLCγ1, and PI3 kinase. The adapter protein SLP-76 is phosphorylated at Tyr113 and Tyr128, allowing for binding of the Grb2-like adapter Gads. Phosphorylation of SLP-76 at Ser376 by hematopoietic progenitor kinase 1 (HPK1) induces interaction with 14-3-3ε and down-regulates TCR signaling (7,8). Phosphoinositide-specific phospholipase PLCγ1 enzyme activity is also stimulated by Zap-70 and Syk phosphorylation on Tyr783, Tyr711, and Tyr1253, resulting in robust PI-4,5-P2 hydrolysis (9).
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