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14511
PhosphoPlus® Tau (Thr181) Antibody Duet
Primary Antibodies

PhosphoPlus® Tau (Thr181) Antibody Duet #14511

IHC-P (paraffin) Image 1

Immunohistochemical analysis of paraffin-embedded human Alzheimer's brain using Phospho-Tau (Thr181) (D9F4G) Rabbit mAb.

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Western Blotting Image 2

Western blot analysis of extracts from mouse and rat brain using Phospho-Tau (Thr181) (D9F4G) Rabbit mAb. The phospho-specificity of Phospho-Tau (Thr181) (D9F4G) Rabbit mAb was verified by peptide blocking using a phosphopeptide or non-phosphopeptide targeting residue Thr181.

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Western Blotting Image 3

Western blot analysis of extracts from various cell lines and tissues using Tau (D1M9X) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

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IHC-P (paraffin) Image 4

Immunohistochemical analysis of paraffin-embedded human Alzheimer's brain using Tau (D1M9X) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

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IHC-P (paraffin) Image 5

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Phospho-Tau (Thr181) (D9F4G) Rabbit mAb.

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IHC-P (paraffin) Image 6

Immunohistochemical analysis of paraffin-embedded human normal appendix using Tau (D1M9X) XP® Rabbit mAb.

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IHC-P (paraffin) Image 7

Immunohistochemical analysis of paraffin-embedded mouse brain using Phospho-Tau (Thr181) (D9F4G) Rabbit mAb.

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IHC-P (paraffin) Image 8

Immunohistochemical analysis of paraffin-embedded T-47D cell pellet (left, positive) or MDA-MB-231 cell pellet (right, negative) using Tau (D1M9X) XP® Rabbit mAb.

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IHC-P (paraffin) Image 9

Immunohistochemical analysis of paraffin-embedded mouse colon, control (left) or λ phosphatase-treated (right), using Phospho-Tau (Thr181) (D9F4G) Rabbit mAb.

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IHC-P (paraffin) Image 10

Immunohistochemical analysis of paraffin-embedded mouse lung using Tau (D1M9X) XP® Rabbit mAb.

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IF-IC Image 11

Confocal immunofluorescent analysis of T-47D (positive, left) or MDA-MB-231 (negative, right) cells using Tau (D1M9X) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

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IF-F Image 12

Confocal immunofluorescent analysis of mouse striatum (left), hippocampus (middle), or midbrain (right) using Tau (D1M9X) XP® Rabbit mAb (green) and GFAP (GA5) Mouse mAb #3670 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Phospho-Tau (Thr181) (D9F4G) Rabbit mAb 12885 100 µl
  • WB
  • IP
  • IHC
H M R 50-80 Rabbit IgG
Tau (D1M9X) XP® Rabbit mAb 46687 100 µl
  • WB
  • IHC
  • IF
H M R 50 to 80 Rabbit IgG

PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.

Tau is a heterogeneous microtubule-associated protein that promotes and stabilizes microtubule assembly, especially in axons. Six isoforms with different amino-terminal inserts and different numbers of tandem repeats near the carboxy terminus have been identified, and tau is hyperphosphorylated at approximately 25 sites by Erk, GSK-3, and CDK5 (1,2). Phosphorylation decreases the ability of tau to bind to microtubules. Neurofibrillary tangles are a major hallmark of Alzheimer's disease; these tangles are bundles of paired helical filaments composed of hyperphosphorylated tau. In particular, phosphorylation at Ser396 by GSK-3 or CDK5 destabilizes microtubules. Furthermore, research studies have shown that inclusions of tau are found in a number of other neurodegenerative diseases, collectively known as tauopathies (1,3).

The cerebrospinal fluid concentration of Tau phosphorylated at Thr181 has been proposed to be a biomarker for the study of neurodegenerative disorders (4).

  1. Johnson, G.V. and Stoothoff, W.H. (2004) J Cell Sci 117, 5721-9.
  2. Hanger, D.P. et al. (1998) J Neurochem 71, 2465-76.
  3. Bramblett, G.T. et al. (1993) Neuron 10, 1089-99.
  4. Mitchell, A.J. (2009) J Neurol Neurosurg Psychiatry 80, 966-75.
Entrez-Gene Id
4137
Swiss-Prot Acc.
P10636-8
For Research Use Only. Not For Use In Diagnostic Procedures.

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PhosphoPlus is a trademark of Cell Signaling Technology, Inc.
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