Render Target: STATIC
Render Timestamp: 2025-03-19T10:42:07.020Z
Commit: 779953b12a5930618aae6aca7c87fb286faeb1d7
XML generation date: 2025-03-07 13:09:35.613
Product last modified at: 2025-01-01T09:04:56.910Z
Cell Signaling Technology Logo
1% for the planet logo
PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

THEM2 Antibody #42713

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 15
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    THEM2 Antibody recognizes endogenous levels of total THEM2 protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human THEM2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    THEM2 is a homotetrameric fatty acyl–CoA thioesterase (1). THEM2 and PC-TP (phosphatidylcholine transfer protein), both enriched in liver, interact to form a complex (1). Cell membrane-bound phosphatidylcholines bind to PC-TP in the complex (1). The complex in turn inhibits IRS-2 and mTORC1, which leads to the suppression of insulin signaling (1). THEM2 has also been shown to regulate adaptive thermogenesis in mice (2).
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.