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THOC4/ALY Antibody #11824

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Inquiry Info. # 11824

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    Product Specifications

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 27
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    THOC4/ALY Antibody recognizes endogenous levels of total THOC4/ALY protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

    Species predicted to react based on 100% sequence homology:

    Hamster, Xenopus, Zebrafish, Bovine, Dog, Horse, Guinea Pig

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala245 of human THOC4/ALY protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    mRNA export is a process that is tightly coupled to mRNA splicing (1-4). Splicing and packaging of mRNAs in the form of an mRNA-protein complex (mRNP) leads to the recruitment of the mRNA export adaptor THOC4/ALY, via its interaction with the splicing factor UAP56, forming a large complex termed the transcription-export complex (TREX) (1,2,5). THOC4/ALY then directly interacts with NXF1/TAP, a part of the heterodimer that targets the mRNP to the nuclear pore complex, resulting in the shuttling of mRNP out of the nucleus and into the cytoplasm (1-3,6).

    Alternate Names

    Ally of AML-1 and LEF-1; ALY; ALY/REF; Aly/REF export factor; ALYREF; BEF; bZIP enhancing factor; bZIP-enhancing factor BEF; REF; THO complex 4; THO complex subunit 4; Tho4; THOC4; Transcriptional coactivator Aly/REF

    For Research Use Only. Not for Use in Diagnostic Procedures.
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