TIAR (D32D3) XP® Rabbit mAb #8509
- WB
- IP
- IF
- F
- eCLIP
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 42 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
- F-Flow Cytometry
- eCLIP-eCLIP
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Immunofluorescence (Immunocytochemistry) | 1:800 - 1:3200 |
| Flow Cytometry (Fixed/Permeabilized) | 1:400 - 1:1600 |
| eCLIP | 1:200 |
Storage
Protocol
Specificity / Sensitivity
TIAR (D32D3) XP® Rabbit mAb recognizes endogenous levels of total TIAR protein. This antibody also weakly recognizes recombinant TIA1 protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human TIAR protein.
Background
TIAR is a member of the RNA-recognition motif (RRM) family of RNA-binding proteins (1,2). It functions as a translational repressor under conditions of cellular damage (3,4). In response to cellular stress, TIAR associates with eIF1, eIF3, and the 40S ribosomal subunit and forms noncanonical preinitiation complexes that are translationally inactive (3,4). TIAR then aggregates with its family member TIA1 and facilitates the accumulation of the translationally inactive preinitiation complexes into discrete cytoplasmic foci called stress granules. The two major isoforms of TIAR are the products of alternative mRNA splicing (5,6).
- Tian, Q. et al. (1991) Cell 67, 629-39.
- Kawakami, A. et al. (1992) Proc Natl Acad Sci USA 89, 8681-5.
- Anderson, P. and Kedersha, N. (2002) Cell Stress Chaperones 7, 213-21.
- Anderson, P. and Kedersha, N. (2002) J Cell Sci 115, 3227-34.
- Kawakami, A. et al. (1994) J Immunol 152, 4937-45.
- Beck, A.R. et al. (1996) Nucleic Acids Res 24, 3829-35.
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