TIFA Antibody #61358
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 21 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Protocol
Specificity / Sensitivity
TIFA Antibody recognizes endogenous levels of total TIFA protein.
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro159 of human TIFA protein. Antibodies are purified by peptide affinity chromatography.
Background
TRAF-interacting protein with FHA domain-containing protein A (TIFA) is a cytosolic adaptor protein that is a critical node in an innate immune signaling axis that is activated upon detection of proinflammatory stimuli derived from a bacterial pathogen-associated molecular pattern metabolite, ADP-heptose (1-4). ADP-heptose binds and activates Alpha-protein kinase 1, which drives the phosphorylation-dependent oligomerization and activation of TIFA (5,6). Activated TIFA is then poised to shape the innate immune response by triggering TRAF6 oligomerization, IKK activation, and NF-κB-dependent proinflammatory gene transcription (1,2,5,7).
Limited Uses
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