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TIFA (E9W1L) Rabbit Monoclonal Antibody #54939

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  • WB
  • IP

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 21
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    TIFA (E9W1L) Rabbit Monoclonal Antibody recognizes endogenous levels of total TIFA protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro159 of human TIFA protein.

    Background

    TRAF-interacting protein with FHA domain-containing protein A (TIFA) is a cytosolic adaptor protein that is a critical node in an innate immune signaling axis that is activated upon detection of proinflammatory stimuli derived from a bacterial pathogen-associated molecular pattern metabolite, ADP-heptose (1-4). ADP-heptose binds and activates Alpha-protein kinase 1, which drives the phosphorylation-dependent oligomerization and activation of TIFA (5,6). Activated TIFA is then poised to shape the innate immune response by triggering TRAF6 oligomerization, IKK activation, and NF-κB-dependent proinflammatory gene transcription (1,2,5,7).

    Alternate Names

    MGC20791; Putative MAPK-activating protein PM14; Putative NF-kappa-B-activating protein 20; T2BP; T6BP; TIFA; TIFAA; TRAF interacting protein with forkhead associated domain; TRAF-interacting protein with a forkhead-associated domain; TRAF-interacting protein with FHA domain-containing protein A; TRAF-interacting protein with forkhead-associated domain; TRAF2 binding protein; TRAF2-binding protein; TRAF6 binding protein

    For Research Use Only. Not for Use in Diagnostic Procedures.
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