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TMEM115 (F6N4Y) Rabbit Monoclonal Antibody #71812

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  • WB
  • IP

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 35
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    This product was developed by CST® scientists in collaboration with The Michael J. Fox Foundation (MJFF). Our partnership aims to accelerate Parkinson’s disease (PD) research by identifying and developing high-quality monoclonal antibodies for promising PD targets.

    Visit the CST Parkinson’s Disease Resource Center to learn more: https://cst-science.com/PD-Resources.

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    TMEM115 (F6N4Y) Rabbit Monoclonal Antibody recognizes endogenous levels of total TMEM115 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp325 of human TMEM115 protein.

    Background

    Transmembrane protein 115 (TMEM115) is a 35 kDa protein localized to the Golgi cisternae. Both the N- and C-terminal ends of TMEM115 are oriented toward the cytosol, with four transmembrane domains and a Golgi targeting sequence located in between. While the functions of this protein are not fully understood, it is suggested to play roles in Golgi-to-ER retrograde transport and glycosylation (1). Elevated TMEM115 expression levels have been observed in glioma and hepatocellular carcinoma, indicating its potential as a biomarker and therapeutic target for these cancers (2,3). Golgi dysfunction is associated with many diseases, including cancer, neurodegeneration, and cardiovascular diseases. As a Golgi-specific transmembrane protein, TMEM115 can be leveraged for immunoprecipitation experiments to purify and study the Golgi proteome, metabolome, and lipidome in health and disease (4).

    Alternate Names

    PL6; Placental protein 6; PP6; Protein PL6; testicular tissue protein Li 201; TM115; TMEM115; Transmembrane protein 115

    For Research Use Only. Not for Use in Diagnostic Procedures.
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