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9971
Toll-like Receptor Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

Toll-like Receptor Antibody Sampler Kit #9971

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Toll-like Receptor Antibody Sampler Kit: Image 1
Western blot analysis of THP-1 and MUTZ-3 cells using Toll-like Receptor 2 (D7G9Z) Rabbit mAb.
Toll-like Receptor Antibody Sampler Kit: Image 2
Western blot analysis of extracts from HeLa cells, mock transfected or transfected with human TLR1, using Toll-like Receptor 1 Antibody.
Toll-like Receptor Antibody Sampler Kit: Image 3
Western blot analysis of extracts from various cell lines using Toll-like Receptor 7 (D7) Rabbit mAb.
Toll-like Receptor Antibody Sampler Kit: Image 4
Western blot analysis of extracts from various cell lines using Toll-like Receptor 9 (D2C9) Rabbit mAb.
Toll-like Receptor Antibody Sampler Kit: Image 5
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
Toll-like Receptor Antibody Sampler Kit: Image 6
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing HA-tagged full-length human TLR2 (hTLR2-HA; +), using Toll-like Receptor 2 (D7G9Z) Rabbit mAb.
Toll-like Receptor Antibody Sampler Kit: Image 7
Western blot analysis of extracts from SR cells using Toll-like Receptor 1 Antibody.
Toll-like Receptor Antibody Sampler Kit: Image 8
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with human TLR7 (+), using Toll-like Receptor 7 (D7) Rabbit mAb.
Toll-like Receptor Antibody Sampler Kit: Image 9
Immunoprecipitation of TLR2 from MUTZ-3 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Toll-like Receptor 2 (D7G9Z) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Toll-like Receptor 2 (D7G9Z) Rabbit mAb.
To Purchase # 9971
Cat. # Size Qty. Price
9971T
1 Kit  (4 x 20 microliters)
$ 349

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Toll-like Receptor 1 Antibody 2209 20 µl
  • WB
H 86 Rabbit 
Toll-like Receptor 2 (D7G9Z) Rabbit mAb 12276 20 µl
  • WB
  • IP
H 90-105 Rabbit IgG
Toll-like Receptor 7 (D7) Rabbit mAb 5632 20 µl
  • WB
  • IP
H 140 Rabbit IgG
Toll-like Receptor 9 (D2C9) Rabbit mAb 5845 20 µl
  • WB
  • IP
H 130 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 

Product Description

The Toll-Like Receptor Antibody Sampler Kit is an economical way to examine the total protein levels of a number of toll-like receptors. This kit includes enough primary and secondary antibodies to perform two Western blot experiments with each antibody.

Specificity / Sensitivity

All antibodies contained in this kit detect endogenous levels of their respective total TLR protein. Cross reactivity was not detected with other family members at physiological conditions.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide and are purified by protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing animals with recombinant human proteins or synthetic peptides.

Background

Members of the Toll-like receptor (TLR) family, named for the closely related Toll receptor in Drosophila, play a pivotal role in innate immune responses (1-4). TLRs recognize conserved motifs found in various pathogens and mediate defense responses (5-7). Triggering of the TLR pathway leads to the activation of NF-κB and subsequent regulation of immune and inflammatory genes (4). The TLRs and members of the IL-1 receptor family share a conserved stretch of approximately 200 amino acids known as the Toll/Interleukin-1 receptor (TIR) domain (1). Upon activation, TLRs associate with a number of cytoplasmic adaptor proteins containing TIR domains, including myeloid differentiation factor 88 (MyD88), MyD88-adaptor-like/TIR-associated protein (MAL/TIRAP), Toll-receptor-associated activator of interferon (TRIF), and Toll-receptor-associated molecule (TRAM) (8-10). This association leads to the recruitment and activation of IRAK1 and IRAK4, which form a complex with TRAF6 to activate TAK1 and IKK (8,11-14). Activation of IKK leads to the degradation of IκB, which normally maintains NF-κB in an inactive state by sequestering it in the cytoplasm.
  1. Akira, S. (2003) J Biol Chem 278, 38105-8.
  2. Beutler, B. (2004) Nature 430, 257-63.
  3. Dunne, A. and O'Neill, L.A. (2003) Sci STKE 2003, re3.
  4. Medzhitov, R. et al. (1997) Nature 388, 394-7.
  5. Schwandner, R. et al. (1999) J Biol Chem 274, 17406-9.
  6. Takeuchi, O. et al. (1999) Immunity 11, 443-51.
  7. Alexopoulou, L. et al. (2001) Nature 413, 732-8.
  8. Zhang, F.X. et al. (1999) J Biol Chem 274, 7611-4.
  9. Horng, T. et al. (2001) Nat Immunol 2, 835-41.
  10. Oshiumi, H. et al. (2003) Nat Immunol 4, 161-7.
  11. Muzio, M. et al. (1997) Science 278, 1612-5.
  12. Wesche, H. et al. (1997) Immunity 7, 837-47.
  13. Suzuki, N. et al. (2002) Nature 416, 750-6.
  14. Irie, T. et al. (2000) FEBS Lett 467, 160-4.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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