Render Target: STATIC
Render Timestamp: 2024-10-04T10:26:30.201Z
Commit: f04ddd7fea9fb3592f59f61482fcb94610d25cbe
1% for the planet logo
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

TPP1 (D4E2R) Rabbit mAb #14667

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H Mk
    SENSITIVITY Endogenous
    MW (kDa) 58
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    TPP1 (D4E2R) Rabbit mAb recognizes endogenous levels of total TPP1 protein.

    Species Reactivity:

    Human, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu169 of human TPP1 protein.

    Background

    TPP1 is encoded by the the ACD gene, and is one of six core proteins of the shelterin complex (TRF1, TRF2, Rap1, TIN2, POT1 and TPP1) that regulates telomere length and integrity. This nuclear protein complex localizes to telomeres, and protects the natural ends of chromosomes from inappropriate processing by DNA repair pathways (1). TPP1 was identified in screens for proteins that bind TIN2, which is considered to be the central component of the shelterin complex (1). TPP1 contains two protein-protein interaction domains that facilitate shelterin complex function: a carboxy-terminal TIN2-binding domain and a more central POT1-binding domain. Heterodimerization of TPP1 with POT1 promotes binding to single-stranded telomeric DNA, which facilitates telomere elongation and protection by the shelterin complex. The TPP1 protein also contains a TEL patch, a collection of surface amino acids that recruits telomerase and modulates its processivity (2). In addition to playing an important role in normal development (3), TPP1 is implicated in the etiology of selected diseases. For example, mutations in ACD that alter the composition of the TEL patch have been linked to Hoyeraal-Hreidarsson syndrome, a clinically severe form of dyskeratosis congenita characterized by hematopoietic stem cell dysfunction, bone marrow failure, and a predisposition to cancer (4,5).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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