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  3. TRAIL (F8W2G) Rabbit Monoclonal Antibody
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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

TRAIL (F8W2G) Rabbit Monoclonal Antibody #86049

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  • IP

    Product Specifications

    REACTIVITY M
    SENSITIVITY Endogenous
    MW (kDa) 28-30
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    TRAIL (F8W2G) Rabbit Monoclonal Antibody recognizes endogenous levels of total TRAIL protein. This antibody detects full-length TRAIL as well as the proteolytically cleaved 20 kDa form of TRAIL.

    Species Reactivity:

    Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro115 of mouse TRAIL protein.

    Background

    Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL), also referred to as Apo2 ligand, first identified based on its sequence homology to TNF and Fas/Apo ligand is a member of the TNF family of cytokines and either exists as a type II membrane or soluble protein (1,2). TRAIL induces apoptosis in a variety of transformed cell lines and plays a role in anti-tumor and anti-viral immune surveillance (3). TRAIL signals via binding with death receptors DR4 (TRAIL-R1) (4) and DR5 (TRAIL-R2) (5-8) which can trigger apoptosis as well as NF-κB activation (7,9). Death domains on these receptors leads to the recruitment of a death-induced signaling complex (DISC) leading to caspase-8 and subsequent caspase-3 activation. In addition, TRAIL binds with decoy receptors DcR1 (TRAIL-R3) (6,8,10,11) and DcR2 (TRAIL-R4, TRUNDD) (12,13) which lack the functional cytoplasmic death domain antagonizing TRAIL-induced apoptosis. Osteoprotegerin (OPG) has also been identified as receptor capable of inhibiting TRAIL-induced apoptosis (14). The selectivity of soluble TRAIL at triggering apoptosis in transformed cells as compared to normal cells has led to its investigation as a potential cancer therapeutic (15,16).
    1. Wiley, S.R. et al. (1995) Immunity 3, 673-82.
    2. Pitti, R.M. et al. (1996) J Biol Chem 271, 12687-90.
    3. Almasan, A. and Ashkenazi, A. Cytokine Growth Factor Rev 14, 337-48.
    4. Pan, G. et al. (1997) Science 276, 111-3.
    5. Walczak, H. et al. (1997) EMBO J 16, 5386-97.
    6. MacFarlane, M. et al. (1997) J Biol Chem 272, 25417-20.
    7. Chaudhary, P.M. et al. (1997) Immunity 7, 821-30.
    8. Schneider, P. et al. (1997) FEBS Lett 416, 329-34.
    9. Shetty, S. et al. (2002) Apoptosis 7, 413-20.
    10. Sheridan, J.P. et al. (1997) Science 277, 818-21.
    11. Degli-Esposti, M.A. et al. (1997) J Exp Med 186, 1165-70.
    12. Pan, G. et al. (1998) FEBS Lett 424, 41-5.
    13. Marsters, S.A. et al. (1997) Curr Biol 7, 1003-6.
    14. Kelley, S.K. et al. (2001) J Pharmacol Exp Ther 299, 31-8.
    15. Walczak, H. et al. (1999) Nat Med 5, 157-63.
    16. Ashkenazi, A. et al. (1999) J Clin Invest 104, 155-62.

    Alternate Names

    A330042I21Rik; AI448571; APO-2L; CD253; Ly81; Protein TRAIL; TL2; TNF-related apoptosis inducing ligand; TNF-related apoptosis-inducing ligand; TNF10; Tnfsf10; Tnlg6a; Trail; TRAIL/APO2L; tumor necrosis factor (ligand) superfamily, member 10; tumor necrosis factor ligand 6a; Tumor necrosis factor ligand superfamily member 10

    Database Links

    UniProt ID: P50592


    Entrez-Gene Id: 22035


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