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13405
TRAP1/HSP75 Antibody

TRAP1/HSP75 Antibody #13405

This product is discontinued

We recommend the following alternatives

  • WB
  • IP
  • IF
H
Storage:

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

TRAP1/HSP75 Antibody recognizes endogenous levels of total TRAP1 protein.

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu150 of human TRAP1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

TNF receptor-associated protein 1 (TRAP1), also known as HSP75, is a mitochondrial chaperone and ATPase that was originally identified as a protein that interacts with the TNF receptor. Although a member of the HSP90 family, TRAP1 is not heat-inducible but is upregulated by glucose deprivation, oxidative injury, and UV irradiation. An amino-terminal mitochondrial localization sequence results in localization of TRAP1 within mitochondria (1). Overexpression of TRAP1 decreases oxidative stress, suggesting a protective role in ischemia injury (2). Research studies demonstrate that silencing of TRAP1 enhances cytochrome C release and apoptosis, with additional evidence indicating that TRAP1 can protect cells from cell death by inhibiting the generation of reactive oxygen species (3). TRAP1 is a substrate of the mitochondrial serine/threonine kinase PINK1, whose corresponding gene is mutated in some forms of early-onset Parkinson's disease (PD). PINK1 protects cells from oxidative stress-induced cell death by suppressing release of cytochrome C from mitochondria. PD-linked PINK1 mutations impair the ability of PINK1 to phosphorylate TRAP1 and leads to impaired cell survival (4). Finally, TRAP1 alleviates α-synuclein induced toxicity and rescues the PINK1 loss-of-function phenotype (5).

  1. Felts, S.J. et al. (2000) J Biol Chem 275, 3305-12.
  2. Hua, G. et al. (2007) J Biol Chem 282, 20553-60.
  3. Voloboueva, L.A. et al. (2008) J Cereb Blood Flow Metab 28, 1009-16.
  4. Pridgeon, J.W. et al. (2007) PLoS Biol 5, e172.
  5. Butler, E.K. et al. (2012) PLoS Genet 8, e1002488.
Entrez-Gene Id
10131
Swiss-Prot Acc.
Q12931
For Research Use Only. Not For Use In Diagnostic Procedures.

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