Upstream / Downstream

pathwayImage

Explore pathways related to this product.

Questions?

Find answers on our FAQs page.

ANSWERS  

PhosphoSitePlus® Resource

  • Additional protein information
  • Analytical tools

LEARN MORE

We recommend the following alternatives

W IP   IF     H
REACTIVITY SENSITIVITY MW (kDa) SOURCE
75 Rabbit

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

TRAP1/HSP75 Antibody recognizes endogenous levels of total TRAP1 protein.


Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu150 of human TRAP1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

TNF receptor-associated protein 1 (TRAP1), also known as HSP75, is a mitochondrial chaperone and ATPase that was originally identified as a protein that interacts with the TNF receptor. Although a member of the HSP90 family, TRAP1 is not heat-inducible but is upregulated by glucose deprivation, oxidative injury, and UV irradiation. An amino-terminal mitochondrial localization sequence results in localization of TRAP1 within mitochondria (1). Overexpression of TRAP1 decreases oxidative stress, suggesting a protective role in ischemia injury (2). Research studies demonstrate that silencing of TRAP1 enhances cytochrome C release and apoptosis, with additional evidence indicating that TRAP1 can protect cells from cell death by inhibiting the generation of reactive oxygen species (3). TRAP1 is a substrate of the mitochondrial serine/threonine kinase PINK1, whose corresponding gene is mutated in some forms of early-onset Parkinson's disease (PD). PINK1 protects cells from oxidative stress-induced cell death by suppressing release of cytochrome C from mitochondria. PD-linked PINK1 mutations impair the ability of PINK1 to phosphorylate TRAP1 and leads to impaired cell survival (4). Finally, TRAP1 alleviates α-synuclein induced toxicity and rescues the PINK1 loss-of-function phenotype (5).


1.  Felts, S.J. et al. (2000) J Biol Chem 275, 3305-12.

2.  Hua, G. et al. (2007) J Biol Chem 282, 20553-60.

3.  Voloboueva, L.A. et al. (2008) J Cereb Blood Flow Metab 28, 1009-16.

4.  Pridgeon, J.W. et al. (2007) PLoS Biol 5, e172.

5.  Butler, E.K. et al. (2012) PLoS Genet 8, e1002488.


Entrez-Gene Id 10131
Swiss-Prot Acc. Q12931


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Tween is a registered trademark of ICI Americas, Inc.

13405
TRAP1/HSP75 Antibody