Flow cytometric analysis of human peripheral blood mononuclear cells, untreated (blue) or treated with LPS (300 ng/ml, 16 hr) #14011 (green), using TREM1 (D7D6I) Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype control #3900 (dashed lines) co-stained with a CD14 antibody #36377. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. Analysis was performed on CD14+ cells in the monocyte gate.
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
NOTE: When including fluorescent cellular dyes in your experiment (including viability dyes, DNA dyes, etc.), please refer to the dye product page for the recommended protocol. Visit www.cellsignal.com for a full listing of cellular dyes validated for use in flow cytometry.
NOTE: Optimal centrifugation conditions will vary depending upon cell type and reagent volume. Generally, 150-300g for 1-5 minutes will be sufficient to pellet the cells.
NOTE: If live cell staining is desired, proceed to Section C.
NOTE: Count cells using a hemocytometer or alternative method.
posted January 2009
revised June 2020
Protocol Id: 133
TREM1 (D7D6I) Rabbit mAb recognizes endogenous levels of total TREM1 protein.
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracellular domain of human TREM1 protein.
The triggering receptor expressed on myeloid cells 1 (TREM1) protein is an innate immune receptor that is primarily expressed on the cell surface of myeloid cells (1). TREM1 is a single-pass type I membrane glycoprotein that consists of an extracellular immunoglobulin-like domain, a transmembrane domain, and a cytoplasmic tail. TREM1, like its related protein TREM2, interacts with the tyrosine kinase-binding protein DAP12 to form a receptor-signaling complex (2). By accepting a diverse array of ligands, TREM1-expressing macrophages and neutrophils modulate inflammation through cytokine, chemokine, and receptor upregulation (2,3).
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Alexa Fluor is a registered trademark of Life Technologies Corporation.