Render Target: STATIC
Render Timestamp: 2024-12-13T12:25:38.636Z
Commit: 611277b6de3cd1bb065350b6ef8d63df412b7185
XML generation date: 2024-08-01 15:26:23.820
Product last modified at: 2024-07-03T22:15:07.772Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

TRF2 (P449) Antibody #14824

Filter:
  • WB

    Supporting Data

    REACTIVITY H Mk
    SENSITIVITY Endogenous
    MW (kDa) 65
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    TRF2 (P449) Antibody recognizes endogenous levels of total TRF2 protein. This antibody also cross-reacts with an unidentified protein of 135 kDa.

    Species Reactivity:

    Human, Monkey

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro449 of human TRF2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Telomeres, the linear ends of chromosomes, are organized into T-loops to prevent them from being recognized by the cell as DNA double stranded breaks (DSBs) (1). The telomeric repeat binding factor proteins TRF1 and TRF2 bind to double-stranded telomeres to allow formation of T-loops (2). A large number of proteins involved in the DNA damage response are found physically associated with TRF2 within telomeres (3). Interestingly, TRF2 can transiently localize to DNA damage-induced DSBs, but overexpression of TRF2 prevents ATM-dependent signaling (4). Phosphorylation of TRF2 at Ser323 has been reported in vivo, but no upstream kinase or role has been established for this phosphorylation site (5).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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