TRIF Antibody #4596
- WB
- IP
Supporting Data
REACTIVITY | H |
SENSITIVITY | Endogenous |
MW (kDa) | 98 |
SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:50 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
TRIF (also termed TICAM-1) is a TIR-domain adaptor protein described to activate NF-κB, IRF3 and trigger IFN-β production (4,5). Studies using dominant negative forms of TRIF and siRNA targeting TRIF show that TRIF functions downstream of both TLR3 and TLR4 in response to dsRNA and LPS respectively (4-6). TRIF recruits TRAF6-TAK1-TAB2 to the receptor complex which leads to NF-κB activation (7). In addition, TRIF can trigger signaling of that lead to the induction of apoptosis (8).
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- Dunne, A. and O'Neill, L.A. (2003) Sci STKE 2003, re3.
- Medzhitov, R. et al. (1997) Nature 388, 394-7.
- Schwandner, R. et al. (1999) J Biol Chem 274, 17406-9.
- Takeuchi, O. et al. (1999) Immunity 11, 443-51.
- Alexopoulou, L. et al. (2001) Nature 413, 732-8.
- Zhang, F.X. et al. (1999) J Biol Chem 274, 7611-4.
- Horng, T. et al. (2001) Nat Immunol 2, 835-41.
- Oshiumi, H. et al. (2003) Nat Immunol 4, 161-7.
- Muzio, M. et al. (1997) Science 278, 1612-5.
- Wesche, H. et al. (1997) Immunity 7, 837-47.
- Suzuki, N. et al. (2002) Nature 416, 750-6.
- Irie, T. et al. (2000) FEBS Lett 467, 160-4.
- Yamamoto, M. et al. (2002) J Immunol 169, 6668-72.
- Oshiumi, H. et al. (2003) Nat Immunol 4, 161-7.
- Fitzgerald, K.A. et al. (2003) J Exp Med 198, 1043-55.
- Jiang, Z. et al. (2004) Proc Natl Acad Sci USA 101, 3533-8.
- Kaiser, W.J. and Offermann, M.K. (2005) J Immunol 174, 4942-52.
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