|H Mk||Endogenous||56||Mouse IgG3|
For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
NOTE: Please refer to primary antibody datasheet or product webpage for recommended antibody dilution.
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm).
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
* Avoid repeated exposure to skin.
posted June 2005
revised November 2013
Reprobing of an existing membrane is a convenient means to immunoblot for multiple proteins independently when only a limited amount of sample is available. It should be noted that for the best possible results a fresh blot is always recommended. Reprobing can be a valuable method but with each reprobing of a blot there is potential for increased background signal. Additionally, it is recommended that you verify the removal of the first antibody complex prior to reprobing so that signal attributed to binding of the new antibody is not leftover signal from the first immunoblotting experiment. This can be done by re-exposing the blot to ECL reagents and making sure there is no signal prior to adding the next primary antibody.
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalently purified water.
posted June 2005
revised June 2016
Protocol Id: 262
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
TRXR2/TXNRD2 (3F2-E12-F10) Mouse mAb recognizes endogenous levels of total TRXR2/TXNRD2 protein.
Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the carboxy terminus of human TRXR2/TXNRD2 protein.
Thioredoxin reductases are selenoproteins that are critical for maintaining cellular redox balance and eliminating reactive oxygen species (ROS) (1-4). There are three known mammalian thioredoxin reductases: TRXR1/TXNRD1 (cytosolic), TRXR2/TXNRD2 (mitochondrial), and TRXR3/TXNRD3 (testis-specific) (1). TRXR2 may function to protect against tumor necrosis factor-α (TNF-α)-mediated ROS generation (5). TRXR2 is critical for normal heart development and function (1,4). TRXR2 KO mice experience embryonic lethality, while mitochondrial dysfunction seen in heart-specific TRXR2 KO mice results in congestive heart failure (4). In humans, certain rare mutations in TRXR2 have been linked to dilated cardiomyopathy (1). A recent study in the EPIC-Heidelberg cohort shows that serum selenium levels, in concert with certain SNPs in TRXR2 and other selenoproteins, can influence prostate cancer risk (6).
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|12029S||100 µl (10 western blots)||$ 255.0|