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  3. UBASH3B (E7Y2R) Rabbit Monoclonal Antibody
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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

UBASH3B (E7Y2R) Rabbit Monoclonal Antibody #27599

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  • WB
  • IP

    Product Specifications

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 70
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    UBASH3B (E7Y2R) Rabbit Monoclonal Antibody recognizes endogenous levels of total UBASH3B protein.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human UBASH3B protein.

    Background

    Ubiquitin-associated and SH3 domain-containing protein B (UBASH3B) is a ubiquitously expressed protein that negatively regulates signaling cascades governed by multiple tyrosine kinases. UBASH3B is a modular protein that contains an N-terminal UBA domain that can interact with ubiquitin, a central SH3 domain that mediates protein-protein interactions, and an evolutionarily conserved C-terminal phosphatase domain. Indeed, research studies have shown that UBASH3B interacts with Bcr-Abl and negatively regulates its kinase activity through a mechanism involving dephosphorylation (1). UBASH3B also plays a pivotal role in the adaptive immune response by negatively regulating the activity of key proximal signaling proteins downstream of the T cell receptor (TCR) (2-4). Research studies have also positioned UBASH3B as an oncogenic protein in the context of triple-negative breast cancer, where its overexpression promotes enhanced EGFR stability via protection from c-Cbl-mediated ubiquitination (5,6).

    Alternate Names

    Cbl-interacting protein p70; Cbl-interacting protein Sts-1; KIAA1959; MGC15437; nm23-phosphorylated unknown substrate; p70; SH3 domain-containing 70 kDa protein, suppressor of T-cell receptor signaling 1, nm23-phosphorylated unknown substrate; STS-1; STS1; Suppressor of T-cell receptor signaling 1; T-cell ubiquitin ligand 2; TULA-2; TULA2; Tyrosine-protein phosphatase STS1/TULA2; UBASH3B; ubiquitin associated and SH3 domain containing B; ubiquitin associated and SH3 domain containing, B; Ubiquitin-associated and SH3 domain-containing protein B; UBS3B

    Database Links

    UniProt ID: Q8TF42


    Entrez-Gene Id: 84959


    Limited Uses

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    For Research Use Only. Not for Use in Diagnostic Procedures.
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    KARPAS cell line source: Dr. Abraham Karpas at the University of Cambridge.
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