UBE2G2 (D8Z4G) Rabbit mAb #63182
- WB
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 19 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Source / Purification
Background
The ubiquitin-conjugating enzyme E2 G2 (UBE2G2, UBC7) is a ubiquitously expressed E2 enzyme and critical component of the endoplasmic reticulum-associated degradation pathway (ERAD) (4). Research studies demonstrate that UBE2G2 forms homodimers and preassembles K48-linked poly-Ub chains at its active site (5-8). The association of Ub-charged UBE2G2 molecules with the ER-resident E3 ligase AMFR (gp78) is required for Ub chain transfer and efficient removal of misfolded or aggregated proteins through the ERAD pathway (9,10).
- Ciechanover, A. (1998) EMBO J 17, 7151-60.
- Hochstrasser, M. (2000) Nat Cell Biol 2, E153-7.
- Hochstrasser, M. (2000) Science 289, 563-4.
- Katsanis, N. and Fisher, E.M. (1998) Genomics 51, 128-31.
- Bazirgan, O.A. and Hampton, R.Y. (2008) J Biol Chem 283, 12797-810.
- Ravid, T. and Hochstrasser, M. (2007) Nat Cell Biol 9, 422-7.
- Liu, W. et al. (2014) EMBO J 33, 46-61.
- Li, W. et al. (2007) Nature 446, 333-7.
- Li, W. et al. (2009) Proc Natl Acad Sci U S A 106, 3722-7.
- Chen, B. et al. (2006) Proc Natl Acad Sci U S A 103, 341-6.
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