Render Target: STATIC
Render Timestamp: 2024-11-08T09:47:26.160Z
Commit: 3c1f305a63297e594ac8d7bb5424007d592d68be
XML generation date: 2024-09-30 01:59:26.289
Product last modified at: 2024-09-30T08:00:18.404Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

UBQLN4 (E7N4Q) Rabbit mAb #93988

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 75
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    UBQLN4 (E7N4Q) Rabbit mAb recognizes endogenous levels of total UBQLN4 protein. This antibody cross-reacts with a 150 kDa protein of unknown identity in some lysates and a 95 kDa protein of unknown identity in some rat lysates.

    Species Reactivity:

    Human, Mouse, Rat

    The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

    Species predicted to react based on 100% sequence homology:

    Dog, Horse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys87 of human UBQLN4 protein.

    Background

    Ubiquilin 4 (UBQLN4), a member of the ubiquitin-like (UBL) protein family, mediates protein degradation by binding polyubiquitinated targets as well as the S5a subunit of the proteasome (1,2). UBQLN4 acts as a repressor of homologous recombination-mediated DNA double-strand break repair and drives non-homologous end joining when overexpressed (3). Its role in DNA double-strand break repair is dependent on Ser318, which is phosphorylated by ATM in response to DNA damage (3). UBQLN4 expression is upregulated in colorectal cancer, and high expression is associated with decreased disease-free survival in several other cancers, including neuroblastoma, melanoma, and ovarian cancer (3,4). UBQLN4 has also been shown to directly associate with LC3 and UBQLN1, recruiting UBQLN1 to autophagosomes (5).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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