Render Target: STATIC
Render Timestamp: 2025-03-21T11:17:51.214Z
Commit: 779953b12a5930618aae6aca7c87fb286faeb1d7
XML generation date: 2025-03-07 13:19:17.591
Product last modified at: 2025-02-18T15:30:11.277Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

USP22 (F4M4V) Rabbit mAb #52293

Filter:
  • WB
  • IF

    Supporting Data

    REACTIVITY H Mk
    SENSITIVITY Endogenous
    MW (kDa) 60
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunofluorescence (Immunocytochemistry) 1:100 - 1:200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    USP22 (F4M4V) Rabbit mAb recognizes endogenous levels of total USP22 protein.

    Species Reactivity:

    Human, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu457 of human USP22 protein.

    Background

    Ubiquitin-specific-processing protease 22 (USP22) is a deubiquitinating enzyme that, along with TXN7, ATXN7L3, and ENY2, forms the deubiquitination module of the human SAGA complex (1,2). SAGA is capable of deubiquitinating H2A and H2B in opposition to polycomb activity (3,4). In addition to histone targets, USP22 deubiquitinates and stabilizes SirT1, which in turn deacetylates and suppresses p53 to repress apoptosis (5). Regulation of SirT1 by USP22 has also been shown to affect STAT signaling (6). USP22 plays a role in double-stranded break repair and antibody class switching (7,8). USP22 has also been shown to occupy the SOX2 promoter, blocking transcription and thus regulating pluripotency (9). Many cancers have elevated levels of USP22, which can promote cancer progression and metastasis (10-12).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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