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VAMP3 (F1G2Z) Rabbit Monoclonal Antibody #96837

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    Product Specifications

    REACTIVITY M R
    SENSITIVITY Endogenous
    MW (kDa) 13
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Simple Western™ 1:10 - 1:50
    Immunoprecipitation 1:50
    Immunofluorescence (Frozen) 1:1200
    Immunofluorescence (Immunocytochemistry) 1:1200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    VAMP3 (F1G2Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total VAMP3 protein.

    Species Reactivity:

    Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of mouse VAMP3 protein.

    Background

    Proteins in the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex are integral membrane proteins involved in vesicle transport and membrane fusion by pairing of vesicular SNAREs (v-SNAREs) with cognate target SNAREs (t-SNAREs) (reviewed in 1,2). Vesicle-associated membrane protein 3 (VAMP3), also known as cellubrevin, has a broad tissue distribution and localizes to endosomal compartments (3). VAMP3 interacts with the t-SNAREs syntaxin1, syntaxin4, SNAP23, and SNAP25 (4,5). Research studies indicate that VAMP3 is involved in transferrin receptor recycling to the plasma membrane (6) and T cell receptor recycling to immunological synapses (7). Inhibition of VAMP3 with tetanus toxin impairs membrane trafficking during cell migration (8).

    Alternate Names

    CEB; cellu; Cellubrevin; D130027G05Rik; OTTMUSP00000010900; RP23-455G15.3; Syb3; Synaptobrevin-3; VAM; VAMP-3; Vamp3; Vesicle-associated membrane protein 3

    For Research Use Only. Not for Use in Diagnostic Procedures.
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