Render Target: STATIC
Render Timestamp: 2024-10-14T09:43:36.192Z
Commit: 56767fe525c928647c8401233a175d0d607d385d
XML generation date: 2024-08-01 15:25:54.287
Product last modified at: 2024-09-27T21:00:09.363Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

VDAC Antibody #4866

Filter:
  • WB
  • IHC

    Supporting Data

    REACTIVITY H M R B
    SENSITIVITY Endogenous
    MW (kDa) 32
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IHC-Immunohistochemistry 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • B-Bovine 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunohistochemistry (Paraffin) 1:75

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    VDAC Antibody detects endogenous levels of total VDAC protein.

    Species Reactivity:

    Human, Mouse, Rat, Bovine

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of human VDAC-1. Antibodies are purified using protein A and peptide affinity chromatography.

    Background

    Voltage-dependent anion channel (VDAC), ubiquitously expressed and located in the outer mitochondrial membrane, is generally thought to be the primary means by which metabolites diffuse in and out of the mitochondria (1). In addition, this channel plays a role in apoptotic signaling. The change in mitochondrial permeability characteristic of apoptosis is mediated by Bcl-2 family proteins, which bind to VDAC, altering the channel kinetics (2). Homodimerization of VDAC may be a mechanism for changing mitochondrial permeability and supporting release of cytochrome c (3). In mammalian cells, there are three VDAC isoforms, VDAC1, which is the most widely expressed isoform, as well as VDAC2 and VDAC3 (4,5).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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