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Render Timestamp: 2024-10-11T09:46:00.962Z
Commit: 56767fe525c928647c8401233a175d0d607d385d
XML generation date: 2024-09-30 01:56:23.376
Product last modified at: 2024-09-30T08:00:39.433Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. VPS29 (D2H7G) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

VPS29 (D2H7G) Rabbit mAb #73540

Filter:
  • WB

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 21
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    VPS29 (D2H7G) Rabbit mAb recognizes endogenous levels of total VPS29 protein.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala121 of human VPS29 protein.

    Background

    Retromer is a heteropentameric protein complex that consists of two protein compounds, a sortin-nexin dimer and a trimeric VPS26-VPS29-VPS35 protein subcomplex. The retromer complex associates with endosomes at their cytosolic side to mediate retrograde transport of transmembrane proteins from endosomes to the trans-Golgi network (1-3). Vacuolar protein sorting-associated protein 29 (VPS29) is considered a cryptic metallophosphoesterase, as it contains a conserved metallophosphoesterase-fold that includes a phenylalanine in place of an essential histidine residue within the active site (4). While VPS29 is capable of binding metal ions, it does so with low affinity and exhibits no enzymatic activity. Instead, VPS29 serves as a scaffold protein that interacts with the carboxy-terminal region of VPS35 and is essential for association of the retromer with other endosomal transport proteins (5).

    Database Links

    UniProt ID: Q9UBQ0


    Entrez-Gene Id: 51699


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    For Research Use Only. Not For Use In Diagnostic Procedures.
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