Render Target: STATIC
Render Timestamp: 2024-12-13T11:25:17.703Z
Commit: 611277b6de3cd1bb065350b6ef8d63df412b7185
XML generation date: 2024-09-30 01:53:49.232
Product last modified at: 2024-11-03T13:30:08.007Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

XPF (D3G8C) Rabbit mAb #13465

Filter:
  • WB

    Supporting Data

    REACTIVITY H R Mk
    SENSITIVITY Endogenous
    MW (kDa) 104
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    XPF (D3G8C) Rabbit mAb recognizes endogenous levels of total XPF (ERCC4) protein.

    Species Reactivity:

    Human, Rat, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg670 of human XPF (ERCC4) protein.

    Background

    DNA repair systems operate in all living cells to manage a variety of DNA lesions. Nucleotide excision repair (NER) is implemented in cases where bulky helix-distorting lesions occur, such as those brought about by UV and certain chemicals (1). Excision Repair Cross Complementing 4 (ERCC4, XPF) forms a complex with the ERCC1 excision repair protein to create an essential 5’ endonuclease responsible for lesion excision (2). ERCC1-XPF is also required for repair of DNA interstrand crosslinks (ICLs) (3) and involved in repair of double strand breaks (4). Mutations in the corresponding XPF gene cause specific forms of xeroderma pigmentosum, Cockayne syndrome, and Fanconi anemia, while altered XPF protein levels may be associated with disease progression and response to treatment in specific human cancers (5,6).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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