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56674
YAP/TAZ Transcriptional Targets Antibody Sampler Kit

YAP/TAZ Transcriptional Targets Antibody Sampler Kit #56674

Western Blotting Image 1

Western blot analysis of extracts from various cell lines using CTGF (D8Z8U) Rabbit mAb (upper) and β-actin (D6A8) Rabbit mAb #8457 (lower). Asterisk indicates a 120 kDa band of unknown identity detected by this antibody in Western blot analysis.

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Western Blotting Image 2

Western blot analysis of extracts from various cell lines using CYR61 (D4H5D) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). As expected, MCF7 cells are negative for CYR61 expression.

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Western Blotting Image 3

Western blot analysis of extracts from concentrated culture medium of various cell lines using IGFBP3 (D1U9C) Rabbit mAb.

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Western Blotting Image 4

Western blot analysis of extracts from various cell lines using Integrin β2 (D4N5Z) Rabbit mAb (upper) or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).

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Western Blotting Image 5

Western blot analysis of lysates from Raji (human), BaF3 (mouse) and C6 (rat) cell lines using Survivin (71G4B7E) Rabbit mAb.

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Western Blotting Image 6

Western blot analysis of extracts from various cell lines using Axl (C89E7) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

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Western Blotting Image 7

Western blot analysis of extracts from COS-7 cells transfected with a construct expressing either Myc/DDK-tagged full-length human TEAD1 (hTEAD1-Myc/DDK;+), Myc/DDK-tagged full-length human TEAD2 (hTEAD2-Myc/DDK;+), Myc/DDK-tagged full-length human TEAD3 (hTEAD3-Myc/DDK;+), or Myc/DDK-tagged full-length human TEAD4 (hTEAD4-Myc/DDK;+), using TEAD1 (D9X2L) Rabbit mAb #12292 (A), TEAD2 Antibody #8870 (B), TEAD3 Antibody (C), or Pan-TEAD (D3F7L) Rabbit mAb #13295 (D).

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Western Blotting Image 8

Western blot analysis of extracts from various cell lines using Lamin B2 (D8P3U) Rabbit mAb.

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Western Blotting Image 9

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

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IF-IC Image 10

Confocal immunofluorescent analysis of PANC-1 cells (high-expressing) and MCF7 cells (low-expressing) using CTGF (D8Z8U) Rabbit mAb (green) and an Anti-Na+/K+ ATPase α Mouse mAb (blue). Red = Propidium Iodide (PI)/RNase Staining Solution #4087.

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Flow Cytometry Image 11

Flow cytometric analysis of 293T cells (blue) and PANC-1 cells (green) using CYR61 (D4H5D) XP® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

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IP Image 12

Immunoprecipitation of IGFBP3 protein from HCC1937 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is IGFBP3 (D1U9C) Rabbit mAb. Western blot analysis was performed using IGFBP3 (D1U9C) Rabbit mAb.

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Western Blotting Image 13

Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence® Survivin siRNA II (+), using Survivin (71G4B7E) Rabbit mAb #2808 and α-Tubulin (11H10) Rabbit mAb #2125. Survivin (71G4B7E) Rabbit mAb confirms silencing of survivin expression and α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of survivin siRNA.

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IHC-P (paraffin) Image 14

Immunohistochemical analysis of paraffin-embedded breast carcinoma using Axl (C89E7) Rabbit mAb. Note staining of infiltrating cells.

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Western Blotting Image 15

Western blot analysis of extracts from COS-7 cells transfected with a construct expressing either Myc/DDK-tagged full-length human TEAD1 (hTEAD1-Myc/DDK;+), Myc/DDK-tagged full-length human TEAD2 (hTEAD2-Myc/DDK;+), Myc/DDK-tagged full-length human TEAD3 (hTEAD3-Myc/DDK;+), or Myc/DDK-tagged full-length human TEAD4 (hTEAD4-Myc/DDK;+), using Pan-TEAD (D3F7L) Rabbit mAb.

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IP Image 16

Immunoprecipitation of lamin B2 from Jurkat cell extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Lamin B2 (D8P3U) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Lamin B2 (D8P3U) Rabbit mAb.

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IF-IC Image 17

Confocal immunofluorescent analysis of Saos-2 cells, untreated (left) or treated with Brefeldin A #9972 (10 μg/ml, overnight; center), and untreated MCF7 cells (right), using CYR61 (D4H5D) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4804 (fluorescent DNA dye).

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IHC-P (paraffin) Image 18

Immunohistochemical analysis of paraffin-embedded human transitional epithelial carcinoma of the bladder using Survivin (71G4B7E) Rabbit mAb.

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IHC-P (paraffin) Image 19

Immunohistochemical analysis of paraffin-embedded metastatic lung carcinoma using Axl (C89E7) Rabbit mAb.

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Western Blotting Image 20

Western blot analysis of extracts from various cell lines using Pan-TEAD (D3F7L) Rabbit mAb.

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IF-IC Image 21

Confocal immunofluorescent analysis of HCT 116 cells using Lamin B2 (D8P3U) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

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IHC-P (paraffin) Image 22

Immunohistochemical analysis of paraffin-embedded human lung carcinoma showing nuclear localization using Survivin (71G4B7E) Rabbit mAb #2808.

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IHC-P (paraffin) Image 23

Immunohistochemical analysis of paraffin-embedded cell pellets, NCI-H1299 (left) or Jurkat (right), using Axl (C89E7) Rabbit mAb.

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IP Image 24

Immunoprecipitation of TEAD proteins from COS-7 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Pan-TEAD (D3F7L) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Pan-TEAD (D3F7L) Rabbit mAb.

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IHC-P (paraffin) Image 25

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Survivin (71G4B7E) Rabbit mAb in the presence of control peptide (left) or Survivin Blocking Peptide #1037 (right).

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Flow Cytometry Image 26

Flow cytometric analysis of Jurkat cells (blue) and DU145 cells (green) using Axl (C89E7H4) Rabbit mAb.

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IHC-P (paraffin) Image 27

Immunohistochemical analysis of paraffin-embedded human pituitary adenoma using Survivin (71G4B7E) Rabbit mAb.

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IF-IC Image 28

Confocal immunofluorescent analysis of DU 145 (left) and HCC827 (right) cells using Axl (C89E7) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

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IHC-F (frozen) Image 29

Immunohistochemical analysis of frozen H1650 xenograft showing nuclear localization using Survivin (71G4B7E) Rabbit mAb.

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Flow Cytometry Image 30

Flow cytometric analysis of untreated Jurkat cells using Survivin (71G4B7E) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).

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IF-IC Image 31

Confocal immunofluorescent analysis of HeLa cells using Survivin (71G4B7E) Rabbit mAb (green). Mitochondria have been labeled with MitoTracker® Red CMXRos (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
CTGF (D8Z8U) Rabbit mAb 86641 20 µl
  • WB
  • IF
H 35 Rabbit IgG
CYR61 (D4H5D) XP® Rabbit mAb 14479 20 µl
  • WB
  • IF
  • F
H 41 Rabbit IgG
IGFBP3 (D1U9C) Rabbit mAb 25864 20 µl
  • WB
  • IP
H 40 Rabbit IgG
Integrin β2 (D4N5Z) Rabbit mAb 73663 20 µl
  • WB
H 95 Rabbit IgG
Survivin (71G4B7) Rabbit mAb 2808 20 µl
  • WB
  • IP
  • IHC
  • IF
  • F
H M R 16 Rabbit IgG
Axl (C89E7) Rabbit mAb 8661 20 µl
  • WB
  • IP
  • IHC
  • IF
  • F
H Mk 138 Rabbit IgG
Pan-TEAD (D3F7L) Rabbit mAb 13295 20 µl
  • WB
  • IP
H M R Mk 50, 53, 55, 60 Rabbit IgG
Lamin B2 (D8P3U) Rabbit mAb 12255 20 µl
  • WB
  • IP
  • IF
H Mk 68 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 

The YAP/TAZ Transcriptional Targets Antibody Sampler Kit provides an economical means of detecting proteins whose transcription is subject to regulation by the transcriptional co-activators YAP and/or TAZ. The kit provides enough antibody to perform two western blot experiments with each primary antibody.

Each antibody in the YAP/TAZ Transcriptional Targets Antibody Sampler Kit detects endogenous levels of its target protein. Pan-TEAD (D3F7L) Rabbit mAb was shown to react with TEAD1, 2, 3 and 4 using gene-specific transfected cell extracts.

Monoclonal antibodies are produced by immunizing rabbits with synthetic peptides corresponding to residues Gly54 of human TEAD1, Val174 of human CTGF, Pro171 of human CYR61, Leu49 of human Integrin β2, Lys435 of human lamin B2, Cys60 of human survivin, and recombinant protein fragments corresponding to human Axl, and the amino terminus of human IGFBP3.

YAP and TAZ (WWTR1) are transcriptional co-activators that play a central role in the Hippo Signaling pathway that regulates cell, tissue and organ growth. Under growth conditions, YAP and TAZ are translocated to the nucleus, where they interact with DNA-binding transcription factors (e.g., Transcriptional Enhanced Activation Domain [TEAD] proteins) to regulate the expression of genes that control fundamental aspects of cell function, such as proliferation and cell survival (1). A number of genes have been experimentally confirmed as targets of transcriptional regulation by YAP and TAZ. These include the extracellular matrix proteins CTGF, CYR61, and integrin β2 (2-4), the inhibitor of apoptosis protein (IAP) survivin (5), the mechano-sensitive nuclear envelope protein Lamin B2 (6), and the oncogenic receptor tyrosine kinase Axl (7).

  1. Holden, J.K. and Cunningham, C.N. (2018) Cancers (Basel) 10, .
  2. Zhang, H. et al. (2009) J Biol Chem 284, 13355-62.
  3. Chan, S.W. et al. (2011) J Biol Chem 286, 7018-26.
  4. Chan, S.W. et al. (2011) Oncogene 30, 600-10.
  5. Zhang, W. et al. (2015) Cancer Res 75, 4450-7.
  6. Zanconato, F. et al. (2015) Nat Cell Biol 17, 1218-27.
  7. Cui, Z.L. et al. Int J Immunopathol Pharmacol 25, 989-1001.
Entrez-Gene Id
558 , 1490 , 3491 , 3486 , 3689 , 84823 , 332 , 7003 , 8463 , 7005 , 7004
Swiss-Prot Acc.
P30530 , P29279 , O00622 , P17936 , P05107 , Q03252 , O15392 , P28347 , Q15562 , Q99594 , Q15561
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.

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