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56674
YAP/TAZ Transcriptional Targets Antibody Sampler Kit
Primary Antibodies

YAP/TAZ Transcriptional Targets Antibody Sampler Kit #56674

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Confocal immunofluorescent analysis of PANC-1 cells (high-expressing) and MCF7 cells (low-expressing) using CTGF (D8Z8U) Rabbit mAb (green) and an Anti-Na+/K+ ATPase α Mouse mAb (blue). Red = Propidium Iodide (PI)/RNase Staining Solution #4087.

Confocal immunofluorescent analysis of Saos-2 cells, untreated (left) or treated with Brefeldin A #9972 (10 μg/ml, overnight; center), and untreated MCF7 cells (right), using CYR61 (D4H5D) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4804 (fluorescent DNA dye).

Immunoprecipitation of IGFBP3 protein from HCC1937 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is IGFBP3 (D1U9C) Rabbit mAb. Western blot analysis was performed using IGFBP3 (D1U9C) Rabbit mAb.

Western blot analysis of extracts from various cell lines using Integrin β2 (D4N5Z) Rabbit mAb (upper) or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).

Flow cytometric analysis of untreated Jurkat cells using Survivin (71G4B7E) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).

Flow cytometric analysis of Jurkat cells (blue) and DU145 cells (green) using Axl (C89E7H4) Rabbit mAb.

Immunoprecipitation of TEAD proteins from COS-7 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Pan-TEAD (D3F7L) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Pan-TEAD (D3F7L) Rabbit mAb.

Confocal immunofluorescent analysis of HCT 116 cells using Lamin B2 (D8P3U) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Western blot analysis of extracts from various cell lines using CTGF (D8Z8U) Rabbit mAb (upper) and β-actin (D6A8) Rabbit mAb #8457 (lower). Asterisk indicates a 120 kDa band of unknown identity detected by this antibody in Western blot analysis.

Western blot analysis of extracts from various cell lines using CYR61 (D4H5D) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). As expected, MCF7 cells are negative for CYR61 expression.

Western blot analysis of extracts from concentrated culture medium of various cell lines using IGFBP3 (D1U9C) Rabbit mAb.

Confocal immunofluorescent analysis of HeLa cells using Survivin (71G4B7E) Rabbit mAb (green). Mitochondria have been labeled with MitoTracker® Red CMXRos (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Confocal immunofluorescent analysis of DU 145 (left) and HCC827 (right) cells using Axl (C89E7) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Western blot analysis of extracts from COS-7 cells transfected with a construct expressing either Myc/DDK-tagged full-length human TEAD1 (hTEAD1-Myc/DDK;+), Myc/DDK-tagged full-length human TEAD2 (hTEAD2-Myc/DDK;+), Myc/DDK-tagged full-length human TEAD3 (hTEAD3-Myc/DDK;+), or Myc/DDK-tagged full-length human TEAD4 (hTEAD4-Myc/DDK;+), using TEAD1 (D9X2L) Rabbit mAb #12292 (A), TEAD2 Antibody #8870 (B), TEAD3 Antibody (C), or Pan-TEAD (D3F7L) Rabbit mAb #13295 (D).

Immunoprecipitation of lamin B2 from Jurkat cell extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Lamin B2 (D8P3U) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Lamin B2 (D8P3U) Rabbit mAb.

Immunohistochemical analysis of frozen H1650 xenograft showing nuclear localization using Survivin (71G4B7E) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded breast carcinoma using Axl (C89E7) Rabbit mAb. Note staining of infiltrating cells.

Western blot analysis of extracts from COS-7 cells transfected with a construct expressing either Myc/DDK-tagged full-length human TEAD1 (hTEAD1-Myc/DDK;+), Myc/DDK-tagged full-length human TEAD2 (hTEAD2-Myc/DDK;+), Myc/DDK-tagged full-length human TEAD3 (hTEAD3-Myc/DDK;+), or Myc/DDK-tagged full-length human TEAD4 (hTEAD4-Myc/DDK;+), using Pan-TEAD (D3F7L) Rabbit mAb.

Western blot analysis of extracts from various cell lines using Lamin B2 (D8P3U) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human transitional epithelial carcinoma of the bladder using Survivin (71G4B7E) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using Axl (C89E7) Rabbit mAb.

Western blot analysis of extracts from various cell lines using Pan-TEAD (D3F7L) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human lung carcinoma showing nuclear localization using Survivin (71G4B7E) Rabbit mAb #2808.

Immunohistochemical analysis of paraffin-embedded human B-cell non-Hodgkin's lymphoma using Axl (C89E7) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Survivin (71G4B7E) Rabbit mAb in the presence of control peptide (left) or Survivin Blocking Peptide #1037 (right).

Immunohistochemical analysis of paraffin-embedded human ovarian serous carcinoma using Axl (C89E7) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human pituitary adenoma using Survivin (71G4B7E) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded metastatic lung carcinoma using Axl (C89E7) Rabbit mAb.

Western blot analysis of lysates from Raji (human), BaF3 (mouse) and C6 (rat) cell lines using Survivin (71G4B7E) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded cell pellets, NCI-H1299 (left) or Jurkat (right), using Axl (C89E7) Rabbit mAb.

Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence® Survivin siRNA II (+), using Survivin (71G4B7E) Rabbit mAb #2808 and α-Tubulin (11H10) Rabbit mAb #2125. Survivin (71G4B7E) Rabbit mAb confirms silencing of survivin expression and α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of survivin siRNA.

Western blot analysis of extracts from various cell lines using Axl (C89E7) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

To Purchase # 56674T
Product # Size Price
56674T
1 Kit  (8 x 20 µl) $ 538

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
CTGF (D8Z8U) Rabbit mAb 86641 20 µl
  • WB
  • IF
H 35 Rabbit IgG
CYR61 (D4H5D) XP® Rabbit mAb 14479 20 µl
  • WB
  • IF
  • F
H 41 Rabbit IgG
IGFBP3 (D1U9C) Rabbit mAb 25864 20 µl
  • WB
  • IP
H 40 Rabbit IgG
Integrin β2 (D4N5Z) Rabbit mAb 73663 20 µl
  • WB
H 95 Rabbit IgG
Survivin (71G4B7) Rabbit mAb 2808 20 µl
  • WB
  • IP
  • IHC
  • IF
  • F
H M R 16 Rabbit IgG
Axl (C89E7) Rabbit mAb 8661 20 µl
  • WB
  • IP
  • IHC
  • IF
  • F
H Mk 138 Rabbit IgG
Pan-TEAD (D3F7L) Rabbit mAb 13295 20 µl
  • WB
  • IP
H M R Mk 50, 53, 55, 60 Rabbit IgG
Lamin B2 (D8P3U) Rabbit mAb 12255 20 µl
  • WB
  • IP
  • IF
H Mk 68 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 

Product Description

The YAP/TAZ Transcriptional Targets Antibody Sampler Kit provides an economical means of detecting proteins whose transcription is subject to regulation by the transcriptional co-activators YAP and/or TAZ. The kit provides enough antibody to perform two western blot experiments with each primary antibody.

Specificity / Sensitivity

Each antibody in the YAP/TAZ Transcriptional Targets Antibody Sampler Kit detects endogenous levels of its target protein. Pan-TEAD (D3F7L) Rabbit mAb was shown to react with TEAD1, 2, 3 and 4 using gene-specific transfected cell extracts.

Source / Purification

Monoclonal antibodies are produced by immunizing rabbits with synthetic peptides corresponding to residues Gly54 of human TEAD1, Val174 of human CTGF, Pro171 of human CYR61, Leu49 of human Integrin β2, Lys435 of human lamin B2, Cys60 of human survivin, and recombinant protein fragments corresponding to human Axl, and the amino terminus of human IGFBP3.

Background

YAP and TAZ (WWTR1) are transcriptional co-activators that play a central role in the Hippo Signaling pathway that regulates cell, tissue and organ growth. Under growth conditions, YAP and TAZ are translocated to the nucleus, where they interact with DNA-binding transcription factors (e.g., Transcriptional Enhanced Activation Domain [TEAD] proteins) to regulate the expression of genes that control fundamental aspects of cell function, such as proliferation and cell survival (1). A number of genes have been experimentally confirmed as targets of transcriptional regulation by YAP and TAZ. These include the extracellular matrix proteins CTGF, CYR61, and integrin β2 (2-4), the inhibitor of apoptosis protein (IAP) survivin (5), the mechano-sensitive nuclear envelope protein Lamin B2 (6), and the oncogenic receptor tyrosine kinase Axl (7).

  1. Holden, J.K. and Cunningham, C.N. (2018) Cancers (Basel) 10, .
  2. Zhang, H. et al. (2009) J Biol Chem 284, 13355-62.
  3. Chan, S.W. et al. (2011) J Biol Chem 286, 7018-26.
  4. Chan, S.W. et al. (2011) Oncogene 30, 600-10.
  5. Zhang, W. et al. (2015) Cancer Res 75, 4450-7.
  6. Zanconato, F. et al. (2015) Nat Cell Biol 17, 1218-27.
  7. Cui, Z.L. et al. Int J Immunopathol Pharmacol 25, 989-1001.

Pathways & Proteins

Explore pathways + proteins related to this product.

For Research Use Only. Not For Use In Diagnostic Procedures.

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