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56674
YAP/TAZ Transcriptional Targets Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

YAP/TAZ Transcriptional Targets Antibody Sampler Kit #56674

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YAP/TAZ Transcriptional Targets Antibody Sampler Kit: Image 1

Confocal immunofluorescent analysis of PANC-1 cells (high-expressing) and MCF7 cells (low-expressing) using CTGF (D8Z8U) Rabbit mAb (green) and an Anti-Na+/K+ ATPase α Mouse mAb (blue). Red = Propidium Iodide (PI)/RNase Staining Solution #4087.

YAP/TAZ Transcriptional Targets Antibody Sampler Kit: Image 2

Flow cytometric analysis of 293T cells (blue) and PANC-1 cells (green) using CYR61 (D4H5D) XP® Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

YAP/TAZ Transcriptional Targets Antibody Sampler Kit: Image 3

Immunoprecipitation of IGFBP3 protein from HCC1937 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is IGFBP3 (D1U9C) Rabbit mAb. Western blot analysis was performed using IGFBP3 (D1U9C) Rabbit mAb.

YAP/TAZ Transcriptional Targets Antibody Sampler Kit: Image 4

Western blot analysis of extracts from various cell lines using Integrin β2 (D4N5Z) Rabbit mAb (upper) or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).

YAP/TAZ Transcriptional Targets Antibody Sampler Kit: Image 5

Flow cytometric analysis of untreated Jurkat cells using Survivin (71G4B7E) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).

YAP/TAZ Transcriptional Targets Antibody Sampler Kit: Image 6

Flow cytometric analysis of fixed and permeabilized Jurkat cells (blue, negative) and DU145 cells (green, positive) using Axl (C89E7H4) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

YAP/TAZ Transcriptional Targets Antibody Sampler Kit: Image 7

Immunoprecipitation of TEAD proteins from COS-7 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Pan-TEAD (D3F7L) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Pan-TEAD (D3F7L) Rabbit mAb.

YAP/TAZ Transcriptional Targets Antibody Sampler Kit: Image 8

Confocal immunofluorescent analysis of HCT 116 cells using Lamin B2 (D8P3U) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

YAP/TAZ Transcriptional Targets Antibody Sampler Kit: Image 9

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

YAP/TAZ Transcriptional Targets Antibody Sampler Kit: Image 10

Western blot analysis of extracts from various cell lines using CTGF (D8Z8U) Rabbit mAb (upper) and β-actin (D6A8) Rabbit mAb #8457 (lower). Asterisk indicates a 120 kDa band of unknown identity detected by this antibody in Western blot analysis.

YAP/TAZ Transcriptional Targets Antibody Sampler Kit: Image 11

Confocal immunofluorescent analysis of Saos-2 cells, untreated (left) or treated with Brefeldin A #9972 (10 μg/ml, overnight; center), and untreated MCF7 cells (right), using CYR61 (D4H5D) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4804 (fluorescent DNA dye).

YAP/TAZ Transcriptional Targets Antibody Sampler Kit: Image 12

Western blot analysis of extracts from concentrated culture medium of various cell lines using IGFBP3 (D1U9C) Rabbit mAb.

YAP/TAZ Transcriptional Targets Antibody Sampler Kit: Image 13

Confocal immunofluorescent analysis of HeLa cells using Survivin (71G4B7E) Rabbit mAb (green). Mitochondria have been labeled with MitoTracker® Red CMXRos (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

YAP/TAZ Transcriptional Targets Antibody Sampler Kit: Image 14

Confocal immunofluorescent analysis of DU 145 (left) and HCC827 (right) cells using Axl (C89E7) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

YAP/TAZ Transcriptional Targets Antibody Sampler Kit: Image 15

Western blot analysis of extracts from COS-7 cells transfected with a construct expressing either Myc/DDK-tagged full-length human TEAD1 (hTEAD1-Myc/DDK;+), Myc/DDK-tagged full-length human TEAD2 (hTEAD2-Myc/DDK;+), Myc/DDK-tagged full-length human TEAD3 (hTEAD3-Myc/DDK;+), or Myc/DDK-tagged full-length human TEAD4 (hTEAD4-Myc/DDK;+), using TEAD1 (D9X2L) Rabbit mAb #12292 (A), TEAD2 Antibody #8870 (B), TEAD3 Antibody (C), or Pan-TEAD (D3F7L) Rabbit mAb #13295 (D).

YAP/TAZ Transcriptional Targets Antibody Sampler Kit: Image 16

Immunoprecipitation of lamin B2 from Jurkat cell extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Lamin B2 (D8P3U) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Lamin B2 (D8P3U) Rabbit mAb.

YAP/TAZ Transcriptional Targets Antibody Sampler Kit: Image 17

Western blot analysis of extracts from various cell lines using CYR61 (D4H5D) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). As expected, MCF7 cells are negative for CYR61 expression.

YAP/TAZ Transcriptional Targets Antibody Sampler Kit: Image 18

Immunohistochemical analysis of frozen H1650 xenograft showing nuclear localization using Survivin (71G4B7E) Rabbit mAb.

YAP/TAZ Transcriptional Targets Antibody Sampler Kit: Image 19

Immunohistochemical analysis of paraffin-embedded breast carcinoma using Axl (C89E7) Rabbit mAb. Note staining of infiltrating cells.

YAP/TAZ Transcriptional Targets Antibody Sampler Kit: Image 20

Western blot analysis of extracts from COS-7 cells transfected with a construct expressing either Myc/DDK-tagged full-length human TEAD1 (hTEAD1-Myc/DDK;+), Myc/DDK-tagged full-length human TEAD2 (hTEAD2-Myc/DDK;+), Myc/DDK-tagged full-length human TEAD3 (hTEAD3-Myc/DDK;+), or Myc/DDK-tagged full-length human TEAD4 (hTEAD4-Myc/DDK;+), using Pan-TEAD (D3F7L) Rabbit mAb.

YAP/TAZ Transcriptional Targets Antibody Sampler Kit: Image 21

Western blot analysis of extracts from various cell lines using Lamin B2 (D8P3U) Rabbit mAb.

YAP/TAZ Transcriptional Targets Antibody Sampler Kit: Image 22

Immunohistochemical analysis of paraffin-embedded human transitional epithelial carcinoma of the bladder using Survivin (71G4B7E) Rabbit mAb.

YAP/TAZ Transcriptional Targets Antibody Sampler Kit: Image 23

Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using Axl (C89E7) Rabbit mAb.

YAP/TAZ Transcriptional Targets Antibody Sampler Kit: Image 24

Western blot analysis of extracts from various cell lines using Pan-TEAD (D3F7L) Rabbit mAb.

YAP/TAZ Transcriptional Targets Antibody Sampler Kit: Image 25

Immunohistochemical analysis of paraffin-embedded human lung carcinoma showing nuclear localization using Survivin (71G4B7E) Rabbit mAb #2808.

YAP/TAZ Transcriptional Targets Antibody Sampler Kit: Image 26

Immunohistochemical analysis of paraffin-embedded human B-cell non-Hodgkin's lymphoma using Axl (C89E7) Rabbit mAb.

YAP/TAZ Transcriptional Targets Antibody Sampler Kit: Image 27

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Survivin (71G4B7E) Rabbit mAb in the presence of control peptide (left) or Survivin Blocking Peptide #1037 (right).

YAP/TAZ Transcriptional Targets Antibody Sampler Kit: Image 28

Immunohistochemical analysis of paraffin-embedded human ovarian serous carcinoma using Axl (C89E7) Rabbit mAb.

YAP/TAZ Transcriptional Targets Antibody Sampler Kit: Image 29

Immunohistochemical analysis of paraffin-embedded human pituitary adenoma using Survivin (71G4B7E) Rabbit mAb.

YAP/TAZ Transcriptional Targets Antibody Sampler Kit: Image 30

Immunohistochemical analysis of paraffin-embedded metastatic lung carcinoma using Axl (C89E7) Rabbit mAb.

YAP/TAZ Transcriptional Targets Antibody Sampler Kit: Image 31

Western blot analysis of lysates from Raji (human), BaF3 (mouse) and C6 (rat) cell lines using Survivin (71G4B7E) Rabbit mAb.

YAP/TAZ Transcriptional Targets Antibody Sampler Kit: Image 32

Immunohistochemical analysis of paraffin-embedded cell pellets, NCI-H1299 (left) or Jurkat (right), using Axl (C89E7) Rabbit mAb.

YAP/TAZ Transcriptional Targets Antibody Sampler Kit: Image 33

Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence® Survivin siRNA II (+), using Survivin (71G4B7E) Rabbit mAb #2808 and α-Tubulin (11H10) Rabbit mAb #2125. Survivin (71G4B7E) Rabbit mAb confirms silencing of survivin expression and α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of survivin siRNA.

YAP/TAZ Transcriptional Targets Antibody Sampler Kit: Image 34

Western blot analysis of HeLa Cell Extracts, untreated (-) or Axl knock-out (+) using Axl (C89E7) Rabbit mAb, #8661 (upper) or GAPDH (D16H11) XP® Rabbit mAb, #5174 (lower).

YAP/TAZ Transcriptional Targets Antibody Sampler Kit: Image 35

Western blot analysis of extracts from various cell lines using Axl (C89E7) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

To Purchase # 56674T
Product # Size Price
56674T
1 Kit  (8 x 20 µl) $ 554

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
CTGF (D8Z8U) Rabbit mAb 86641 20 µl
  • WB
  • IF
H 35 Rabbit IgG
CYR61 (D4H5D) XP® Rabbit mAb 14479 20 µl
  • WB
  • IF
  • F
H 41 Rabbit IgG
IGFBP3 (D1U9C) Rabbit mAb 25864 20 µl
  • WB
  • IP
H 40 Rabbit IgG
Integrin β2 (D4N5Z) Rabbit mAb 73663 20 µl
  • WB
H 95 Rabbit IgG
Survivin (71G4B7) Rabbit mAb 2808 20 µl
  • WB
  • IP
  • IHC
  • IF
  • F
H M R 16 Rabbit IgG
Axl (C89E7) Rabbit mAb 8661 20 µl
  • WB
  • IP
  • IHC
  • IF
  • F
H Mk 138 Rabbit IgG
Pan-TEAD (D3F7L) Rabbit mAb 13295 20 µl
  • WB
  • IP
H M R Mk 50, 53, 55, 60 Rabbit IgG
Lamin B2 (D8P3U) Rabbit mAb 12255 20 µl
  • WB
  • IP
  • IF
H Mk 68 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 

Product Description

The YAP/TAZ Transcriptional Targets Antibody Sampler Kit provides an economical means of detecting proteins whose transcription is subject to regulation by the transcriptional co-activators YAP and/or TAZ. The kit provides enough antibody to perform two western blot experiments with each primary antibody.

Specificity / Sensitivity

Each antibody in the YAP/TAZ Transcriptional Targets Antibody Sampler Kit detects endogenous levels of its target protein. Pan-TEAD (D3F7L) Rabbit mAb was shown to react with TEAD1, 2, 3 and 4 using gene-specific transfected cell extracts.

Source / Purification

Monoclonal antibodies are produced by immunizing rabbits with synthetic peptides corresponding to residues Gly54 of human TEAD1, Val174 of human CTGF, Pro171 of human CYR61, Leu49 of human Integrin β2, Lys435 of human lamin B2, Cys60 of human survivin, and recombinant protein fragments corresponding to human Axl, and the amino terminus of human IGFBP3.

Background

YAP and TAZ (WWTR1) are transcriptional co-activators that play a central role in the Hippo Signaling pathway that regulates cell, tissue and organ growth. Under growth conditions, YAP and TAZ are translocated to the nucleus, where they interact with DNA-binding transcription factors (e.g., Transcriptional Enhanced Activation Domain [TEAD] proteins) to regulate the expression of genes that control fundamental aspects of cell function, such as proliferation and cell survival (1). A number of genes have been experimentally confirmed as targets of transcriptional regulation by YAP and TAZ. These include the extracellular matrix proteins CTGF, CYR61, and integrin β2 (2-4), the inhibitor of apoptosis protein (IAP) survivin (5), the mechano-sensitive nuclear envelope protein Lamin B2 (6), and the oncogenic receptor tyrosine kinase Axl (7).

  1. Holden, J.K. and Cunningham, C.N. (2018) Cancers (Basel) 10, .
  2. Zhang, H. et al. (2009) J Biol Chem 284, 13355-62.
  3. Chan, S.W. et al. (2011) J Biol Chem 286, 7018-26.
  4. Chan, S.W. et al. (2011) Oncogene 30, 600-10.
  5. Zhang, W. et al. (2015) Cancer Res 75, 4450-7.
  6. Zanconato, F. et al. (2015) Nat Cell Biol 17, 1218-27.
  7. Cui, Z.L. et al. Int J Immunopathol Pharmacol 25, 989-1001.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.