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ZAP Antibody #72310

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  • WB

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 100
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    ZAP Antibody recognizes endogenous levels of total ZAP protein. This antibody recognizes ZAP isoform 1.

    Species Reactivity:

    Human

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp699 of human ZAP protein. Antibodies are purified by peptide affinity chromatography.

    Background

    ZAP (Zinc finger CCCH-type antiviral protein 1, ZC3HAV1, PARP13) is an RNA-binding protein which inhibits the replication of certain viruses, including HIV-1, by preventing the accumulation of viral RNA in the cytoplasm (1-5). ZAP binds to specific viral mRNAs through the CCCH zinc finger motifs located on its N terminus, and recruits exosomes and decapping enzymes to degrade the target RNA (3-5). ZAP also interferes with the interaction between eIF4A and eIF4G on target mRNA, causing translational repression (6). Isoform 2, the shorter isoform also known as ZAPS, acts as a stimulator of interferon responses in human cells mediated by the RNA helicase RIG-I as part of the innate antiviral immune response (7).

    Alternate Names

    ADP-ribosyltransferase diphtheria toxin-like 13; ARTD13; CCCH-type zinc finger antiviral protein; DKFZp686F2052; DKFZp686H1869; DKFZp686O19171; FLB6421; FLJ13288; Inactive Poly [ADP-ribose] polymerase 13; MGC48898; PARP13; ZAP; ZC3H2; ZC3HAV1; ZC3HDC2; ZCCHV; Zinc finger antiviral protein; Zinc finger CCCH domain-containing protein 2; zinc finger CCCH type, antiviral 1; Zinc finger CCCH-type antiviral protein 1; zinc finger CCCH-type containing, antiviral 1; zinc finger CCCH-type, antiviral 1

    For Research Use Only. Not for Use in Diagnostic Procedures.
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