Render Target: STATIC
Render Timestamp: 2025-03-21T11:34:25.308Z
Commit: 779953b12a5930618aae6aca7c87fb286faeb1d7
XML generation date: 2025-03-07 13:16:27.143
Product last modified at: 2025-01-01T09:02:40.545Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

ZAP Antibody #72310

Filter:
  • WB

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 100
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    ZAP Antibody recognizes endogenous levels of total ZAP protein. This antibody recognizes ZAP isoform 1.

    Species Reactivity:

    Human

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp699 of human ZAP protein. Antibodies are purified by peptide affinity chromatography.

    Background

    ZAP (Zinc finger CCCH-type antiviral protein 1, ZC3HAV1, PARP13) is an RNA-binding protein which inhibits the replication of certain viruses, including HIV-1, by preventing the accumulation of viral RNA in the cytoplasm (1-5). ZAP binds to specific viral mRNAs through the CCCH zinc finger motifs located on its N terminus, and recruits exosomes and decapping enzymes to degrade the target RNA (3-5). ZAP also interferes with the interaction between eIF4A and eIF4G on target mRNA, causing translational repression (6). Isoform 2, the shorter isoform also known as ZAPS, acts as a stimulator of interferon responses in human cells mediated by the RNA helicase RIG-I as part of the innate antiviral immune response (7).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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