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PTMScan® Wild Type Alpha-Lytic Protease (WaLP)
Proteomic Analysis Products

PTMScan® Wild Type Alpha-Lytic Protease (WaLP) #33036

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Product Usage Information

PTMScan® Wild Type Alpha-Lytic Protease (WaLP) is provided for use with Cell Signaling Technology's patented PTMScan® protocol in the initial protein digestion step. Samples in urea lysis buffer need to be diluted to 2M or less urea prior to addition of WaLP. Dilute the 0.4 mg/ml stock 1:100 for final digest and incubate overnight at 37°C. Aliquot and store at -20ºC. Consult the specific PTMScan® kit and protocol for more details on the appropriate protease before digesting any protein samples.


This product is stable for 12 months when stored at -20°C. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Wild type alpha-lytic protease (WaLP) is a serine endopeptidase that cleaves at the carboxyl terminal side of alanine, serine, threonine, and valine amino acid residues.

Specificity / Sensitivity

Species predicted to react based on 100% sequence homology

All Species Expected

Source / Purification

WaLP was produced recombinantly in E. coli, purified and is provided in 10 mM sodium acetate pH 5.0.


Wild type alpha-lytic protease (WaLP) was first isolated from myxobacterium Lysobacter enzymogenes (1). It is a protease that cleaves after A, S, T, and V residues making it a useful alternative protease for proteomics use. The pro-form of the enzyme is expressed as a 397 amino acid protein which is cleaved to a 198 amino acid mature protein. Numerous studies have been published about the mature form, active site, and catalytic mechanism (2, 3, 4). Based on crystal structure studies there seems to be a strong relation to other pancreatic serine proteases (5). The alternative cleavage specificity of WaLP is especially useful for looking at Proteomics of SUMO modifications as it cleaves the SUMO protein leaving a di-glycine motif that can then be detected using the proprietary SUMO Branch Motif remnant (K-ε-GG) antibody (6).

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not for Use in Diagnostic Procedures.
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To Purchase # 33036
Cat. # Size Qty. Price
100 µg
500 µg  (5 x 100 ug)