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80376
PTMScan® Control Peptides Phospho-Tyrosine
Proteomic Analysis Products
Cell Extracts

PTMScan® Control Peptides Phospho-Tyrosine #80376

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PTMScan® Control Peptides Phospho-Tyrosine: Image 1
Annotated tandem mass spectrum (MS2) of the PTMScan® Control Peptides Phospho-Tyrosine component: IGEGTY(p)GVVY[K].
PTMScan® Control Peptides Phospho-Tyrosine: Image 2
Annotated tandem mass spectrum (MS2) of the PTMScan® Control Peptides Phospho-Tyrosine component: GY(p)VPATI[K].
PTMScan® Control Peptides Phospho-Tyrosine: Image 3
Annotated tandem mass spectrum (MS2) of the PTMScan® Control Peptides Phospho-Tyrosine component: LIEDNEY(p)TA[R].
PTMScan® Control Peptides Phospho-Tyrosine: Image 4
Peptides included in the PTMScan® Control Peptides Phospho-Tyrosine mix.  All peptides are stable-isotope labeled, designated by bracketed R or K, and contain a phosphate group designated by parentheses. 
PTMScan® Control Peptides Phospho-Tyrosine: Image 5
Extracted ion chromatograms of PTMScan® Control Peptides Phospho-Tyrosine added at supplied concentration (1X at 200 fmol) to HCT 116 human colon cancer cell peptides prior to immunoaffinity enrichment using PTMScan® Phospho-Tyrosine Rabbit mAb (P-Tyr-1000) Kit #8803. Desalted peptides were analyzed on Q Exactive mass spectrometer and resolved using a 90 min reversed phase gradient from 7.5% to 32% acetonitrile on a C18 column. The peak corresponding to the specific Control Peptide is marked with retention time and observed precursor mass, with peak height reported as the normalized level (NL) for each row per panel.

Product Usage Information

Use with Cell Signaling Technology’s PTMScan® kit protocol from the Immunoaffinity Purification (IAP) step. Because the optimal amount of PTMScan® Control Peptides Phospho-Tyrosine for each user’s experiments will depend on unique factors, such as mass spectrometer sensitivity, users may dilute these control peptides as needed.

1. Aliquot PTMScan® Control Peptides Phospho-Tyrosine for storage as single-use units at -20°C or proceed to immediate usage.
2. Resuspend sample peptides in the appropriate buffer and volume, e.g., 1.4 mL of PTMScan® IAP Buffer (1X).
3. Clear sample peptides by centrifugation.
4. Transfer clarified sample peptides to tubes containing IAP beads.
5. Add 10 µL of PTMScan® Control Peptides Phospho-Tyrosine to IAP beads and sample peptides and mix well.
6. Continue with PTMScan® or PTMScan® HS workflows at the 2-hour incubation step.
7. Detect PTMScan® Control Peptides Phospho-Tyrosine in the LCMS data file.

Storage

This product is stable for 12 months when stored at -20°C. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

The PTMScan® Control Peptides Phospho-Tyrosine enable quality control of immunoaffinity enrichment performance using PTMScan® or PTMScan® HS workflows.  These synthetic peptides contain a specific post-translational modification (PTM) that can be enriched by the associated PTMScan® or PTMScan® HS immunoaffinity purification (IAP) beads, as well as a stable heavy isotope that can be distinguished from endogenous peptides by the mass spectrometer.
CAS 75-05-8, 76-05-1

Background

Tyrosine phosphorylation plays a key role in cellular signaling (1). Research studies have shown that in cancer, unregulated tyrosine kinase activity can drive malignancy and tumor formation by generating inappropriate proliferation and survival signals (2). Antibodies specific for phospho-tyrosine (3,4) have been invaluable reagents in these studies. The phospho-tyrosine monoclonal antibodies developed by Cell Signaling Technology are exceptionally sensitive tools for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery.
  1. Schlessinger, J. (2000) Cell 103, 211-25.
  2. Blume-Jensen, P. and Hunter, T. (2001) Nature 411, 355-65.
  3. Ward, S.G. et al. (1992) J Biol Chem 267, 23862-9.
  4. Glenney, J.R. et al. (1988) J Immunol Methods 109, 277-85.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
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To Purchase # 80376
Cat. # Size Qty. Price
80376S
100 µl  (10 assays)
$ 200