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Pricing & Additional Information

To learn more about our Proteomics Kits and Services, including pricing, please answer a few questions for our proteomics group.

Contact the CST Proteomics Group  

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PhosphoSitePlus® Resource

  • Additional protein information
  • Analytical tools

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Product Includes Cap Color Volume (with Count)
PTMScan® Phospho-Ser/Thr-Pro Motif [p(S/T)P] Immunoaffinity Beads Blue 10 x 80 µl
PTMScan® IAP Buffer (10X) 9993 White 10 x 600 µl
PTMScan® Limited Use License 1 x  license

Product Usage Information

Cells are lysed in a urea-containing buffer, cellular proteins are digested by proteases, and the resulting peptides are purified by reversed-phase solid-phase extraction. Peptides are then subjected to immunoaffinity purification using a PTMScan® Motif Antibody conjugated to protein A agarose beads. Unbound peptides are removed through washing, and the captured PTM-containing peptides are eluted with dilute acid. Reversed-phase purification is performed on microtips to desalt and separate peptides from antibody prior to concentrating the enriched peptides for LC-MS/MS analysis. CST recommends the use of PTMScan® IAP Buffer #9993 included in the kit. A detailed protocol and Limited Use License allowing the use of the patented PTMScan® method are included with the kit.


Storage: Antibody beads supplied in IAP buffer containing 50% glycerol. Store at -20°C. Do not aliquot the antibody.

Product Description

PTMScan® Technology employs a proprietary methodology from Cell Signaling Technology (CST) for peptide enrichment by immunoprecipitation using a specific bead-conjugated antibody in conjunction with liquid chromatography (LC) tandem mass spectrometry (MS/MS) for quantitative profiling of post-translational modification (PTM) sites in cellular proteins. These include phosphorylation (PhosphoScan®), ubiquitination (UbiScan®), acetylation (AcetylScan®), and methylation (MethylScan®), among others. PTMScan® Technology enables researchers to isolate, identify, and quantitate large numbers of post-translationally modified cellular peptides with a high degree of specificity and sensitivity, providing a global overview of PTMs in cell and tissue samples without preconceived biases about where these modified sites occur. For more information on PTMScan® Proteomics Services, please visit www.cellsignal.com/common/content/content.jsp?id=ptmscan-services.


The CMGC super family of proline-directed protein kinases is a highly conserved and ancient group of proteins that phosphorylate a wide array of substrates involved in stress response, growth and differentiation, and cell division cycle. The consensus sequences targeted by MAPK, SAPK/JNK, CDK, CKII, ERK, and GSK all include a serine or threonine phosphorylation site followed by a proline (1-3). Cell Signaling Technology has developed antibodies that specifically bind to phospho-(S/T)P motifs to enable the study of a large proportion of the phospho-proteome and to identify new substrates of the CMGC kinases.


1.  Pearson, R.B. and Kemp, B.E. (1991) Methods Enzymol 200, 62-81.

2.  Shah, O.J. et al. (2003) J Biol Chem 278, 16433-42.

3.  Gonzalez, F.A. et al. (1991) J Biol Chem 266, 22159-63.



For Research Use Only. Not For Use In Diagnostic Procedures.
AcetylScan is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
MethylScan is a trademark of Cell Signaling Technology, Inc.
PhosphoScan is a trademark of Cell Signaling Technology, Inc.
PTMScan is a trademark of Cell Signaling Technology, Inc.
UbiScan is a trademark of Cell Signaling Technology, Inc.
Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at ptmscan@cellsignal.com.

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PTMScan® Phospho-Ser/Thr-Pro Motif [p(S/T)P] Kit