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72969
PTMScan® Trypsin, Mass Spectrometry Grade
Proteomic Analysis Products

PTMScan® Trypsin, Mass Spectrometry Grade #72969

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Product Usage Information

PTMScan® Trypsin, Mass Spectrometry Grade is provided for use with Cell Signaling Technology's patented PTMScan® protocol in the Trypsin Digestion of Enriched Peptides step. Reconstitute the lyophilized trypsin with 40ul of mass spectrometry grade water to make a 0.5 ug/ul stock. Use immediately or aliquot and store at -80°C. Consult the specific PTMScan® kit and protocol for more details.

Storage:

Store lyophilized trypsin powder at -20ºC protected from moisture. Once reconstituted, store trypsin solutions at -80ºC. Lyophilized trypsin has a shelf life of 1 year at -20ºC and solutions are stable for 6 months at -80ºC.

Product Description

Trypsin is a serine endopeptidase derived from its inactive pancreatic zymogen, trypsinogen, when the N-terminal 6 amino acid leader sequence is enzymatically removed. Activated trypsin cleaves amide and ester bonds of lysine and arginine and this mass spectrophotometry grade Trypsin is intended for use in protein sequencing, and proteomics applications.

Background

Trypsin digests polypeptides by hydrolysis at the carboxyl side of unmodified arginine and lysine residues (1). Proteolysis is slower when the cleavage site is flanked by acidic residues and will not occur if the lysine or arginine is followed by a proline (2-5). Trypsin's activity is optimal at pH 8.0 and is inhibited to varying degrees by organophosphorus compounds such as diisopropyl fluorophosphate, as well as EDTA, aprotinin, Ag+, and benzamidine (6-8).

  1. Brown, W.E. and Wold, F. (1973) Biochemistry 12, 828-34.
  2. Rodriguez, J. et al. (2008) J Proteome Res 7, 300-5.
  3. Leiros, H.K. et al. (2004) Protein Sci 13, 1056-70.
  4. Rawlings, N.D. and Barrett, A.J. (1994) Methods Enzymol 244, 19-61.
  5. Perona, J.J. and Craik, C.S. (1995) Protein Sci 4, 337-60.
  6. KOSTKA, V. and CARPENTER, F.H. (1964) J Biol Chem 239, 1799-803.
  7. Levilliers, N. et al. (1970) Arch Biochem Biophys 140, 474-83.
  8. Polgár, L. (2005) Cell Mol Life Sci 62, 2161-72.
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PTMScan is a trademark of Cell Signaling Technology, Inc.

To Purchase # 72969S
Product # Size Price
72969P
20 µg  (20 ug) $ 25
72969S
100 µg  (5 x 20 µg) $ 110