Store lyophilized trypsin powder at 4ºC protected from moisture. Once reconstituted, store trypsin solutions at -80ºC. Lyophilized trypsin has a shelf life of 1 year at 4º C and solutions are stable for 6 months at -80º C.
Trypsin is chromatographically purified from bovine pancreas, treated with L-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK) to inhibit chymotryptic activity, 0.22 micron diafiltered against 1 mM HCl, and lyophilized.
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