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33036
PTMScan® Wild Type Alpha-Lytic Protease (WaLP)
Proteomic Analysis Products

PTMScan® Wild Type Alpha-Lytic Protease (WaLP) #33036

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Product Usage Information

PTMScan® Wild Type Alpha-Lytic Protease (WaLP) is provided for use with Cell Signaling Technology's patented PTMScan® protocol in the initial protein digestion step. Samples in urea lysis buffer need to be diluted to 2M or less urea prior to addition of WaLP. Dilute the 0.4 mg/ml stock 1:100 for final digest and incubate overnight at 37°C. Aliquot and store at -20ºC. Consult the specific PTMScan® kit and protocol for more details on the appropriate protease before digesting any protein samples.

Storage:

This product is stable for 12 months when stored at -20°C. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Wild type alpha-lytic protease (WaLP) is a serine endopeptidase that cleaves at the carboxyl terminal side of alanine, serine, threonine, and valine amino acid residues.

Background

Wild type alpha-lytic protease (WaLP) was first isolated from myxobacterium Lysobacter enzymogenes (1). It is a protease that cleaves after A, S, T, and V residues making it a useful alternative protease for proteomics use. The pro-form of the enzyme is expressed as a 397 amino acid protein which is cleaved to a 198 amino acid mature protein. Numerous studies have been published about the mature form, active site, and catalytic mechanism (2, 3, 4). Based on crystal structure studies there seems to be a strong relation to other pancreatic serine proteases (5). The alternative cleavage specificity of WaLP is especially useful for looking at Proteomics of SUMO modifications as it cleaves the SUMO protein leaving a di-glycine motif that can then be detected using the proprietary SUMO Branch Motif remnant (K-ε-GG) antibody (6).

  1. Whitaker, DR. The alpha-Lytic Protease. Methods in Enzymology, 19, 599-613
  2. Sohl, JL, et al., , IntramolecularChaperones and Protein Folding, Landes Company, Austin TX (1995) ISBN 978-1570592928 pp. 61-83
  3. Bauer, C.A. et al. (1981) Eur J Biochem 120, 289-94.
  4. Bachovchin, W.W. et al. (1981) Proc Natl Acad Sci U S A 78, 7323-6.
  5. Olson, M.O. et al. (1970) Nature 228, 438-42.
  6. Meyer, J.G. et al. (2014) Mol Cell Proteomics 13, 823-35.

Pathways & Proteins

Explore pathways + proteins related to this product.

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PTMScan is a trademark of Cell Signaling Technology, Inc.

To Purchase # 33036S
Product # Size Price
33036P
400 µg $ 999
33036S
1.6 mg  (4 x 400 µg) $ 3,299