PTMScan® Wild Type Alpha-Lytic Protease (WaLP) is provided for use with Cell Signaling Technology's patented PTMScan® protocol in the initial protein digestion step. Samples in urea lysis buffer need to be diluted to 2M or less urea prior to addition of WaLP. Dilute the 0.4 mg/ml stock 1:100 for final digest and incubate overnight at 37°C. Aliquot and store at -20ºC. Consult the specific PTMScan® kit and protocol for more details on the appropriate protease before digesting any protein samples.
This product is stable for 12 months when stored at -20°C. Aliquot to avoid multiple freeze/thaw cycles.
All Species Expected
WaLP was produced recombinantly in E. coli, purified and is provided in 10 mM sodium acetate pH 5.0.
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