Reconstitute with 1.0 ml of dH20 to create a 0.8 mg/ml working solution. Centrifuge if the solution is not clear. It is recommended to prepare the working solution on day of use.
Recommended Antibody Dilutions:
ELISA (chromogenic substrates): 1:5K-1:100K
Western Blotting (chromogenic substrates): 1:5K - 1:100K
Western Blotting (ECL substrates): 1:10K - 1:200K
Recommended antibody dilutions are provided in ranges because the optimal dilution is dependent on many factors, such as antigen density and permeability.
Anti-Human IgG, Fc gamma Fragment Specific, HRP-Linked Antibody is produced by immunizing goats with human IgG. The Anti-Human IgG, Fc gamma Fragment is purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
Chemiluminescence systems have emerged as the best all-around method for western blot detection. They eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. The speed and sensitivity of these methods are unequalled by traditional alternatives, and because results are generated on film, it is possible to record and store data permanently. Blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies. HRP-conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal. HRP conjugates have a very high turnover rate, yielding good sensitivity with short reaction times.
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