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99339
Human ACE2 (18-652) Recombinant Protein (hFc-Tag)
Recombinant Proteins
Protein Control

Human ACE2 (18-652) Recombinant Protein (hFc-Tag) #99339

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other Image 1 - Human ACE2 (18-652) Recombinant Protein (hFc-Tag)

The purity of Human ACE2 (18-652) Recombinant Protein (hFc-Tag) was determined by densitometry after SDS-PAGE of 2 μg of protein, followed by staining with Coomassie Blue. Purity values were determined from the DTT-reduced samples (+).

Supporting Data

MW (kDa) 150, 300 kDa (non-reduced); 130 kDa (reduced)

Application Key:

  • W-Western
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Formulation

Expression Host: Human (HEK293 cells)

Supplied in a PBS solution (pH 7.2).

Storage

Stable at -80°C for 1 year after receipt. Avoid repeated freeze-thaw cycles.

Product Description

Human ACE2 (18-652) Recombinant Protein (hFc-Tag) is derived from a recombinant expression construct corresponding to the extracellular domain of human ACE2 protein, including the sequence regions that function as receptors for SARS and SARS-CoV-2 coronaviruses. The expressed protein contains a human IgG1 Fc-Tag at its carboxy terminus.

Purity

79%, determined by SDS-PAGE.

Background

ACE2 is a carboxypeptidase that catalyses the conversion of angiotensin I to angiotensin 1-9, or of angiotensin II to the vasodilator angiotensin 1-7 (1). ACE2 is a critical component in the renin-angiotensin system (RAS). ACE2 is predominantly expressed in vascular endothelial cells of the heart and kidney and Leydig and Sertoli cells of the testis (2,3). The unique expression pattern of ACE2 determines its essential role in the regulation of cardiovascular and kidney functions, as well as fertility. ACE2 protein is localized mainly in the extracellular space with its carboxy terminal end attached to the membrane via its transmembrane domain. Active ACE2 enzyme is secreted by cleavage at the amino terminus. Research studies have shown that ACE2 expression is elevated in human failing heart (4). ACE2 has also been identified as the receptor for SARS and SARS-CoV-2 coronaviruses (5-7).

  1. Schmidt, B.L. et al. (2000) J Clin Microbiol 38, 1279-82.
  2. Boehm, M. and Nabel, E.G. (2002) N Engl J Med 347, 1795-7.
  3. Douglas, G.C. et al. (2004) Endocrinology 145, 4703-11.
  4. Goulter, A.B. et al. (2004) BMC Med 2, 19.
  5. Li, W. et al. (2005) EMBO J 24, 1634-43.
  6. Hoffmann, M. et al. (2020) Cell 181, 271-280.e8.
  7. Lan, J. et al. (2020) Nature 581, 215-20.

Pathways & Proteins

Explore pathways + proteins related to this product.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
To Purchase # 99339S
Product # Size Price
99339S
20 µg $ 379