The optimal dilution of the anti-biotin antibody should be determined by titration. However, a final dilution of 1:500 – 1:2000 should yield acceptable results for flow cytometry assays.
Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Monoclonal antibody is produced by immunizing animals with biotinylated KLH.
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