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39681
Anti-biotin (D5A7) Rabbit mAb (PE Conjugate)
Secondary Antibodies
Secondary Antibody

Anti-biotin (D5A7) Rabbit mAb (PE Conjugate) #39681

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Flow Cytometry Image 1: Anti-biotin (D5A7) Rabbit mAb (PE Conjugate)
Flow cytometric analysis of live Jurkat, Clone E6-1 cells engineered to stably express an scFV-based Anti-CD20 CAR using Protein L Biotinylated (green solid line) compared to secondary antibody alone (green dashed line) or unstained cells (orange dashed line). Anti-biotin (D5A7) Rabbit mAb (PE Conjugate) was used as a secondary antibody. Cell line was provided by the Lohmueller Lab, University of Pittsburgh.
To Purchase # 39681
Cat. # Size Qty. Price
39681S
250 µl $ 211

Supporting Data

REACTIVITY All
SENSITIVITY
MW (kDa)
Source/Isotype Rabbit 

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Product Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) dye and tested in-house for detection of biotinylated antibodies for direct flow cytometric analysis.

Product Usage Information

The optimal dilution of the anti-biotin antibody should be determined by titration. However, a final dilution of 1:500 – 1:2000 should yield acceptable results for flow cytometry assays.

Storage

Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

Specificity / Sensitivity

Anti-biotin (D5A7) Rabbit mAb (PE Conjugate) recognizes biotin attached to proteins, peptides, oligonucleotides, and solid matrices.

Species Reactivity:

All Species Expected

Source / Purification

Monoclonal antibody is produced by immunizing animals with biotinylated KLH.

Background

Biotin is a water-soluble B complex vitamin (vitamin H or B7) that serves as a coenzyme in various metabolic functions. It transfers carbon dioxide for five carboxylase enzymes and is involved in the TCA cycle and gluconeogenesis. In addition, all cells require biotin for cell proliferation, production of fatty acids, and metabolism of fats and amino acids (1). Biotin recycling and attachment to histone proteins is catalyzed by an enzyme named biotinidase. Histone biotinylation is involved in regulating telomere attrition and cellular senescence. Biotinidase deficiency is a rare genetic disorder that can cause neurological and cutaneous symptoms if left untreated (2). Biotin has a high affinity for its natural ligand, avidin. The complex and irregular structure of the biotin-binding site makes it highly optimized for biotin binding and confers great specificity to the avidin-biotin complexes (3). This remarkable affinity has been exploited for numerous immunoassays, including ELISA, Flow Cytometry, Immunofluorescence, In Situ Hybridization, Affinity Chromatography, and Immunohistochemistry. Anti-biotin (D5A7) Rabbit mAb #5597 can be used as an alternative to avidins in order to minimize background and maximize signal intensity.
  1. Zempleni, J. et al. (2010) Biofactors 35, 36-46.
  2. Pindolia, K. et al. (2011) Mol Genet Metab 102, 161-9.
  3. Reznik, G.O. et al. (1998) Proc Natl Acad Sci U S A 95, 13525-30.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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