Reconstitute with 1.0 ml of dH20 to create a 0.8 mg/ml working solution. Centrifuge if the solution is not clear. It is recommended to prepare the working solution on day of use.
Recommended Antibody Dilutions:
ELISA (chromogenic substrates): 1:5K-1:100K
Western Blotting (chromogenic substrates): 1:5K - 1:100K
Western Blotting (ECL substrates): 1:10K - 1:200K
Recommended antibody dilutions are provided in ranges because the optimal dilution is dependent on many factors, such as antigen density and permeability.
Supplied lyophilized with a formulation of 0.01M Sodium Phosphate (pH 7.6), 0.25M NaCl, and 15 mg/ml bovine serum albumin (BSA). Store lyophilized at 4ºC. In lyophilized form, the product is stable for 24 months. Once in solution, store at -80ºC or add glycerol (ACS grade or better) to a final concentration of 50% and store at -20ºC. Solutions should be used within 12 months. Aliquot to avoid multiple freeze/thaw cycles.
Anti-Human IgG, Fc gamma Fragment Specific, HRP-Linked Antibody is produced by immunizing goats with human IgG. The Anti-Human IgG, Fc gamma Fragment is purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
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