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14708
Anti-rabbit IgG (H+L), Biotinylated Antibody
Secondary Antibodies
Secondary Antibody

Anti-rabbit IgG (H+L), Biotinylated Antibody #14708

Citations (185)
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  1. WB
Western blot analysis of HeLa and 3T3 cells using α-Actinin (D6F6) XP® Rabbit mAb #6487 (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower), with #14708 as the secondary antibody. Membranes were probed with Streptavidin-HRP #3999 for one hour after secondary incubation in order to detect biotinylated proteins.
To Purchase # 14708
Cat. # Size Qty. Price
14708S
1 ml

Supporting Data

REACTIVITY Rab
SENSITIVITY
MW (kDa)
Source/Isotype Goat 

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • C&R-CUT&RUN
  • C&T-CUT&Tag
  • DB-Dot Blot
  • eCLIP-eCLIP
  • IF-Immunofluorescence
  • F-Flow Cytometry

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • GP-Guinea Pig
  • Rab-Rabbit
  • All-All Species Expected

Product Description

Affinity purified goat anti-rabbit IgG (H+L) antibody is conjugated to biotin. This product has been optimized for use as a secondary antibody in western blotting applications.

Product Usage Information

Recommended Antibody Dilutions:

1:1000-1:3000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Anti-rabbit IgG (H+L), (Biotinylated) reacts with the heavy and light chains of most rabbit immunoglobulins. Less than 1% cross-reactivity is observed with immunoglobulins from other species.

Species Reactivity:

Rabbit

Background

Biotinylated anti-species IgG conjugated antibodies enable signal amplification and are ideal for detecting lower expressing proteins.

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not for Use in Diagnostic Procedures.
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