Anti-rabbit IgG (H+L) (DyLight 680 Conjugate) #5366
- WB
Supporting Data
| REACTIVITY | Rab |
| SENSITIVITY | |
| MW (kDa) | |
| Source/Isotype | Goat |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- Rab-Rabbit
Product Information
Product Description
Product Usage Information
The optimal dilution of the anti-species antibody should be determined by the user. However, the final dilutions below should yield acceptable results for the respective applications.
Fluorescent western blotting: 1:15000
In-Cell Western: 1:500
Storage
Protocol
Specificity / Sensitivity
Anti-rabbit IgG (H+L) (DyLight 680 Conjugate) reacts with heavy and light chain of most rabbit immunoglobulins. No cross-reactivity to other serum proteins has been detected. This antibody may cross-react with immunoglobulins from other species.
Species Reactivity:
Rabbit
Source / Purification
This antibody is prepared from goat antibodies and purified by immunoaffinity chromatography using antigen coupled to agarose beads.
Background
Near infrared anti-species IgG conjugates are ideal for fluorescent western blotting and In-Cell Western. Cell Signaling Technology's strict quality control procedures assure that each conjugate provides optimal specificity and fluorescence.
This product has been optimized for use as a secondary antibody in fluorescent western blotting and In-Cell Western™.
Limited Uses
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