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Render Timestamp: 2024-07-26T09:59:34.349Z
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PDP - Template Name: Secondary Antibody
PDP - Template ID: *******76815a7

Anti-rabbit IgG (H+L) (DyLight 800 4X PEG Conjugate) #5151

Filter:
  • WB

    Supporting Data

    REACTIVITY Rab
    SENSITIVITY
    MW (kDa)
    Source/Isotype Goat
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • Rab-Rabbit 

    Product Information

    Product Description

    Anti-rabbit IgG (H+L) was conjugated to DyLight 800 4X PEG fluorescent dye under optimal conditions and formulated at 1 mg/ml. Excitation is 777 nm and peak fluorescence emission is 794 nm.

    Product Usage Information

    The optimal dilution of the anti-species antibody should be determined by the user. However, the final dilutions below should yield acceptable results for the respective applications.

    Fluorescent western blotting: 1:30000

    In-Cell Western: 1:1000

    Storage

    Proprietary buffer. Store at 4°C. Protect from Light. Do not freeze.

    Protocol

    Specificity / Sensitivity

    Anti-rabbit IgG (H+L) (DyLight 800 4X PEG Conjugate) reacts with heavy and light chain of most rabbit immunoglobulins. No cross-reactivity to other serum proteins has been detected. This antibody may cross-react with immunoglobulins from other species.


    Species Reactivity:

    Rabbit

    Source / Purification

    This antibody is prepared from goat antibodies and purified by immunoaffinity chromatography using antigen coupled to agarose beads.

    Background

    Near infrared anti-species IgG conjugates are ideal for fluorescent western blotting and In-Cell Western. Cell Signaling Technology's strict quality control procedures assure that each conjugate provides optimal specificity and fluorescence.
    This product has been optimized for use as a secondary antibody in fluorescent western blotting and In-Cell Western.

      For Research Use Only. Not For Use In Diagnostic Procedures.
      Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
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