Render Target: STATIC
Render Timestamp: 2024-12-06T10:55:12.041Z
Commit: 224419269841c11382c4555dbee545259bf6c379
XML generation date: 2024-09-20 06:18:39.645
Product last modified at: 2024-11-26T17:45:10.656Z
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PDP - Template Name: Secondary Antibody
PDP - Template ID: *******76815a7

Anti-rabbit IgG (H+L), F(ab')2 Fragment (PE Conjugate) #79408

Filter:
  • F

    Supporting Data

    REACTIVITY Rab
    SENSITIVITY
    MW (kDa)
    Source/Isotype Goat
    Application Key:
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • Rab-Rabbit 

    Product Information

    Product Description

    Anti-rabbit IgG (H+L), F(ab')2 Fragment was conjugated to phycoerythrin (PE) under optimal conditions. This F(ab')2 fragment product results in less non-specific binding, as it lacks the Fc domain that can bind to the cells with Fc receptors.

    Product Usage Information

    The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution of 1:250 - 1:1000 should yield acceptable results for flow cytometry assays.

    Storage

    Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

    Specificity / Sensitivity

    F(ab')2 fragments are prepared from goat antibodies that have been affinity purified and shown no reactivity against non-immunoglobulin rabbit serum proteins, serum proteins from bovine, human, or mouse and IgG from human or mouse based on immunoelectrophoresis (IEP) testing.

    Species Reactivity:

    Rabbit

    Background

    This product has been optimized for use as a secondary antibody in flow cytometry. Fluorescent anti-species IgG conjugates are ideal for flow cytometry and immunofluorescence. Cell Signaling Technology’s strict quality control procedures assure that each conjugate provides optimal specificity and fluorescence.
      For Research Use Only. Not For Use In Diagnostic Procedures.
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