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8885
Anti-rabbit IgG (H+L), F(ab')2 Fragment (PE Conjugate)

Anti-rabbit IgG (H+L), F(ab')2 Fragment (PE Conjugate) #8885

APPLICATIONS

Flow Cytometry

Flow cytometric analysis of Jurkat cells, untreated (green) or treated with LY294002 #9901, Wortmannin #9951, and U0126 #9903 (blue), using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060 detected with Anti-rabbit IgG (H+L), F(ab')2 Fragment (PE Conjugate).

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The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution of 1:250 - 1:1000 should yield acceptable results for flow cytometry assays.

Storage:

Supplied in 0.1 M sodium phosphate, 0.1 M sodium chloride, pH 7.5, 5 mM sodium azide. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

Anti-rabbit IgG (H+L), F(ab')2 Fragment was conjugated to phycoerythrin (PE) under optimal conditions and formulated at 1 mg/ml. This F(ab')2 fragment results in less non-specific binding to cells through Fc receptors.

F(ab')2 fragments are prepared from goat antibodies that have been adsorbed against pooled human serum, mouse serum, plasmacytoma/hybridoma proteins, and purified human paraproteins.

This product has been optimized for use as a secondary antibody in FLOW cytometry. Fluorescent anti-species IgG conjugates are ideal for flow cytometry and immunofluorescence. Cell Signaling Technology’s strict quality control procedures assure that each conjugate provides optimal specificity and fluorescence.

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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To Purchase # 8885S

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Product # Size Price
8885S
250 µl $ 199.0