1. Equilibrate SignalStain® Boost IHC Detection Reagent (AP, Mouse) to room temperature prior to applying to the slides.
2. Cover sections with 1-3 drops (40-120 µl), as needed, and incubate in a humidified chamber for 30 minutes at room temperature.
3. Wash slides and develop with any alkaline phosphatase-compatible substrate.
SignalStain® Boost IHC Detection Reagent (AP, Mouse) should be stored at 4°C and is stable for 12 months.
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