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8114
SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)
Secondary Antibodies

SignalStain® Boost IHC Detection Reagent (HRP, Rabbit) #8114

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  1. IHC

Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma using Sox2 (D6D9) XP® Rabbit mAb #3579 with biotin-based detection (left) or SignalStain® Boost IHC Detection Reagent (HRP, Rabbit) (right).

Immunohistochemical analysis of paraffin-embedded human papillary renal cell carcinoma using Phospho-Met (Tyr1234/1235) (D26) XP® Rabbit mAb #3077 with biotin-based detection (left) or SignalStain® Boost IHC Detection Reagent (HRP, Rabbit) (right).

To Purchase # 8114S
Product # Size Price
8114P
1 ml $ 39
8114S
15 ml $ 367

Supporting Data

REACTIVITY
SENSITIVITY
MW (kDa)
Isotype Goat 

Application Key:

  • W-Western
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Product Description

SignalStain® Boost IHC Detection Reagent (HRP, Rabbit) is a ready-to-use solution intended for use in immunohistochemical assays to detect rabbit primary antibodies. SignalStain® Boost IHC Detection Reagent (HRP, Rabbit) is a highly sensitive, one-step, polymer-based detection reagent that is specific for rabbit IgG. It can be used to visualize targets in both paraffin-embedded and frozen tissue sections and it is compatible with all peroxidase-based substrates.

Product Usage Information

Equilibrate SignalStain® Boost IHC Detection Reagent (HRP, Rabbit) to room temperature prior to applying to the slides. Cover sections with 1-3 drops (40-120 µl), as needed, and incubate in a humidified chamber for 30 minutes at room temperature. Wash slides and develop with any peroxidase-based substrate.

Storage:

SignalStain® Boost IHC Detection Reagent (HRP, Rabbit) should be stored at 4°C and is stable for 12 months.

Specificity / Sensitivity

SignalStain® Boost IHC Detection Reagent (HRP, Rabbit) can be used to detect rabbit primary antibodies in immunohistochemical assays. Some antibodies may require titration when used with this highly sensitive reagent.

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
SignalStain is a trademark of Cell Signaling Technology, Inc.